油松雌性不育性与胚珠发育过程中物质代谢关系的初探

Relationship between metabolism in developmental ovule and female-sterile in Pinus tabulaeformis Carr

该文在不育系雌配子体休眠解除期(Ⅰ)、游离核停止分裂期(Ⅱ)、败育期(Ⅲ)时,取油松可育系和不育系胚珠、珠鳞及由胚珠诱导的悬浮细胞为材料,比较了它们在重量(w)、可溶性糖(ss)、游离氨基酸(aa)和可溶性蛋白质(sp)含量上的变化.结果表明:不育系连体胚珠在Ⅰ期,ss、aa和sp低于可育系;Ⅱ期,ss、aa高于可育系,sp低于可育系;到Ⅲ期,只有aa高于可育系.不育系连体胚珠在3个发育时期,w和aa呈上升趋势,ss和sp分别呈下降趋势.而可育系连体胚珠的鲜重(fw)、sp呈上升趋势,ss、aa呈下降趋势.在相同营养物质和植物激素条件下,相应连体胚珠发育时期离体培养的两系胚珠和悬浮细胞在fws、s、aas、p含量的变化上呈现与可育系连体胚珠相似的趋势,但不育系的fw、sp均高于可育系,ssa、a均低于可育系.不同育性和3个发育时期的连体及离体胚珠的fws、s、aa、sp差异极显著,不同育性悬浮培养细胞的ss、aas、p差异极显著.珠鳞的发育与胚珠的发育表现出相关性.我们认为,不育系胚珠内营养物质和植物激素在含量上的不足或配比上的不协调,使胚珠在ss、aa和sp代谢上出现紊乱,可能导致了不育系胚珠的败育.

Contents of soluble sugared（ss）,free amino acid（aa）,solube protein（sp） and weight（w） in ovule,ovulliferous scale and the suspended culture cell,which was induced from the ovules of female sterile line and fertile line of Pinus tabulaeformis Carr.,were compared at the stages of ovule dormancy breaking（Ⅰ）,free nuclei gametophytes stopping division（Ⅱ） and ovule aborted（Ⅲ） of sterile line.The results showed that the contents of ss,aa and sp in sterile line ovule were all lower than those in fertile line at the Ⅰ period;ss as well as aa were higher and sp was lower than those in fertile line at the Ⅱ period;at the Ⅲ period,only aa was higher than that in fertile line.In the process of ovule development,w and the contents of aa in sterile line were increasing,while ss and sp were decreasing.There was an upward trend in the contents of fresh weight（fw） and sp in fertile line,and a downward trend in the contents of ss and aa.According to the developmental stages,on the conditions of same nutrient material and phytohormone,at the corresponding developing periods,the changing patterns of the contents（fw,ss,aa,sp） in vitro ovules and the suspended culture cell were similar to those in ovules of the fertile line.The contents of fw and sp of sterile line were higher and ss together with aa were lower than those in fertile line in the three development periods.Sterile line and fertile line at their three developing stages had distinct differences on the contents of fw,ss,aa and sp and had striking dissimilarity on the contents of ss,aa and sp.The development of ovuliferous and ovule showed correlationship.It is concluded that the turbulence of metabolism of ss,aa and sp made by insufficient content and absonant partnership of nutrient material and phytohormone of ovule may be the reason of ovule abortion in the female-sterile line of P.tabulaeformis.