高速逆流色谱分离紫苏酮的研究

Separation of perilla ketone by high speed countercurrent chromatography.

紫苏酮是一种对试验动物肺部具有潜在毒性的物质.该文采用高速逆流色谱分离法从紫苏叶中分离出了紫苏酮.结果表明,以V(正己烷)∶V(乙醇)∶V(水)=6∶5∶1为溶剂系统,上相为固定相,下相为流动相,转速为800 r/min,流动相流速为1.2 mL/min,进样1 g粗提物,6 h后分离出28.0 mg紫苏酮,经气相色谱测定,组分纯度达到98.8%,并用核磁共振等方法进行了结构鉴定.

Perilla ketone is a potential lung toxin to laboratory-used animals.A new method for preparative separation of perilla ketone from the leaves of Perilla frutescens by high speed countercurrent chromatography（HSCCC） was established to evaluate the possible hazards to human health.The results showed that HSCCC was an efficient tool in the separation of perilla ketone.A mixture of hexane,ethanol and water in the ratio of 6∶5∶1 was used as the solvent system,which was shaken in a separatory funnel,and after settling for phase separation,the upper one was taken as the stationary phase,and the lower one as the mobile phase.The mobile phase was operated at a flow rate of 1.2 mL/min,while the apparatus rotated at 800 r/min.After the proceeding separation for 6 hours,28.0 mg of perilla ketone was obtained with a purity of 98.8 % determined by gas chromatography in one step elution from 1 g crude extract,and the identification was performed by nuclear magnetic resonance（^1H-NMR、^13C-NMR and DEPT-NMR）.