细胞外信号调节激酶1/2途径在血小板衍生生长因子诱导的大鼠心脏成纤维细胞增殖与胶原合成中的调节作用

Mediating effect of extracellular signal-regulated kinase 1/2 in PDGF-induced proliferation and collagen synthesis of cultured rat cardiac fibroblasts

目的:探讨血小板衍生生长因子(PDGF)是否通过细胞外信号调节激酶1/2(ERK1/2)途径调节大鼠心脏成纤维细胞增殖和胶原的合成。方法:建立新生大鼠心脏成纤维细胞系,MTT法检测心脏成纤维细胞代谢活性的改变,流式细胞仪法检测细胞周期变化,免疫细胞化学法检测Ⅰ、Ⅲ型胶原表达的改变,Western印迹法检测ERK1/2及Phospho-ERK1/2表达的改变。结果:在PDGF刺激下,大鼠心脏成纤维细胞代谢活性增强,细胞增殖指数(PI)增加,Ⅰ、Ⅲ型胶原表达增加,ERK1/2表达无明显改变,而Phospho-ERK1/2表达增强;25μmol/LPD98059预孵育1h抑制PDGF的以上刺激作用。结论:PDGF能够通过ERK1/2途径调节大鼠心脏成纤维细胞的增殖和胶原合成。

Objective： To investigate whether PDGF induces proliferation and collagen synthesis in rat cardiac fibroblasts （CFb） by extracellular signal-regulated klnase 1/2 （ERK 1/2） activation. Methods： Neonatal rat cardiac fibroblasts were isolated. Metabolic activity was observed by MTT. Cell cycle was detected by flow cytometry. Expressions of typeⅠ and type Ⅲcollagen were measured by immunocytochemistry and expressions of phospho-ERK 1/2 and ERK 1/2 were detected by Western blot. Results：Compared with the control group, PDGF increased metabolic activi- ty, proliferous index （PI）, expressions of type Ⅰand type Ⅲcollagen and phosphorylation of ERK 1/2, while expressions of ERK 1/2 were not changed. However, PD98059 inhibited these stimulations of PDGF. Conclusion： ERK 1/2 pathway is involved in PDGF-induced proliferation and collagen synthesis of cultured rat cardiac fibroblasts.