摘要
目的:研究海人酸(kainic acid,KA)侧脑室注射并诱发癫痫持续状态(status epilepticus,SE)后大鼠海马结构中noggin基因在大鼠海马的表达变化。方法:大鼠在侧脑室注射KA后1、3、7、14、30和60d等不同时间,采用RT-PCR研究noggin mRNA含量变化,采用免疫组化观察noggin蛋白表达变化。结果:在正常大鼠海马结构中noggin mRNA有少量表达。noggin阳性细胞主要位于齿状回及CA3、CA1区,数量较少。侧脑室注射KA诱发SE后,noggin表达持续升高,3d达到高峰;7d在脑内的表达开始降低;注射后2个月,noggin表达降至术前水平,仅见散在的阳性细胞。结论:侧脑室注射KA并诱发SE后,大鼠海马结构中noggin表达明显增加。
Objective： To observe the expression of noggin in hippocampal formation of adult rats after status epilepticus （SE） induced by kainic acid（KA）. Methods： Adult male SD rats were randomly divided into a control group and an experimental group. The rats in the experimental group were infused with 1 gl（0. 5μg/μl） KA into the lateral ventricle and sacrificed at 1,3,7,14,30, and 60 d after infusion. The expression of noggin was detected by immunohistochemical and RT-PCR methods. Results： The rats in the control group showed low expression of noggin mRNA in the hippocampal formation. The noggin positive cells in the control group were scattered in dentate gyrus and CA3,CA1 of hippocampus. The expression of noggin in the experimental group increased after SE and reached to peak on the third day, and then decreased gradually and to base level 2 month later. Conclusion： The expression of noggin in rat hippocampus may increase after SE is induced by KA.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2007年第4期442-444,448,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金(30500148
30571770)
