Tpc1808对H_2O_2诱导的C6细胞氧化应激性损伤的保护作用

Protective effects of Tpc1808 on rat C6 glioma cells damage induced by oxidative stress

目的:研究Tpc1808对H2O2诱导的C6细胞氧化应激性损伤的保护作用。方法:采用细胞培养、基因转染、MTT、LDH、免疫荧光组织化学、Western印迹等技术,以星形胶质瘤C6细胞为研究对象,以H2O2作为氧化应激刺激物,建立细胞损伤模型。结果:当终浓度为100μmol/L的H2O2作用于C6细胞24h后,MTT减小,LDH增加,细胞出现明显凋亡;转染Tpc1808基因的细胞受到保护,grp75表达量增加,与对照组相比有显著差异。结论:Tpc1808基因可以保护H2O2引起的C6细胞氧化应激性损伤,该保护作用可能是通过增加grp75表达实现的。

Objective：To investigate the protective effects of Tpc1808 gene on hydrogen-peroxide（H202 ）-induced cell apoptosis in rat glioma C6 cells. Methods： MTT assay, LDH release assay and Hoechest stained were used. Results： H202 addition reduced the viability of C6 cells and increased the LDH release, but these were prevented by Tpc1808 pre-expressing in C6 cells. C6 cells expressing Tpc1808 significantly decreased apoptosis ratio and apoptosis bodies induced by H2O2 according to FCM analysis using PI as a fluorescent bye and Hoechst staining. Additionally, Tpc1808 induced grp75 protein expression in H2O2-induced C6 cells. Conclusion： These data indicate that Tpc1808 may inhibit ROS-mediated cytotoxic effects through induction of grp75 protein expression.