银杏酮酯对大鼠坐骨神经损伤后生长相关蛋白-43表达的影响

Effect of extract of Ginkgo Biloba leaves (EGb50) on the expression of GAP-43 protein after sciatic nerve injury in rats

目的:研究银杏酮酯对大鼠坐骨神经损伤后生长相关蛋白43(GAP-43)表达的影响。方法:SD大鼠78只,随机分成正常组、损伤对照组与实验组,给予不同处理,后两组切断右侧坐骨神经并缝合。实验组给予银杏酮酯200mg·kg-1.d-1溶于1ml生理盐水中灌胃,损伤对照组给予生理盐水1ml灌胃,正常组不做处理。分别于术后1、3、7、14、21及28d取吻合口远段的神经、相应节段的脊神经节及脊髓,应用免疫组织化学和图像分析的方法研究所取组织中GAP-43蛋白的表达并进行定量分析。结果:实验组坐骨神经、脊神经节及脊髓中GAP-43蛋白免疫阳性区域面积和平均光密度值在术后7、14和21d明显高于对照组。结论:大鼠坐骨神经损伤后用银杏酮酯治疗,在早期可促使坐骨神经及相应节段脊神经节和脊髓组织中的GAP-43蛋白表达增加。

Objective： To investigate the effect of extract of Ginkgo Biloba leaves（EGbs0） on the expression of GAP-43 after sciatic nerve injuriy in rats. Methods： Sciatic nerve injury model was established by transacting the right sciatic nerve in 72 male SD rats,which were randomly divided into two groups. The rats were fed with EGb50 in the experimental group and with normal saline in the control group. At the same time,other 6 rats were employed as the normal control without any treatments. The distal part of the injuried nerve, dorsal root ganglia and spinal cord were taken on the 1,3,7,14,21 and 28 days after operation. Immunohistochemistry and image analysis were done to evaluate the expression of GAP-43. Results：The expression of GAP-43 in the sciatic nerve, dorsal root ganglia and spinal cord were higher in the experimental group than that in the control group at 7,14 and 21 days（P〈0. 05,P〈0. 01）. Conclusion：EGb50 can increase the expression of GAP-43 after sciatic nerve injury in rats.