白血病抑制因子在子痫前期患者胎盘组织中的表达

Expression of Leukemia Inhibitory Factor in Preeclampsia Placenta

目的:测定白血病抑制因子(LIF)在子痫前期患者胎盘组织中的表达,探讨其在妊娠高血压发病中的作用。方法:采用免疫组化技术-链霉菌抗生物素蛋白-过氧化酶连接(SP)法对在正常妊娠(正常妊娠组)、子痫前期(轻度子痫前期组和重度子痫前期组)孕妇胎盘组织中LIF的表达进行组织学定位和半定量分析。结果:3组孕妇胎盘组织中LIF均呈现阳性表达,阳性染色主要位于滋养细胞胞质中,胞核无明显着色。轻度子痫前期组和重度子痫前期组分别与正常妊娠组比较,胎盘组织的染色强度及范围显著减弱,差异有统计学意义(P<0.01),而轻度子痫前期组和重度子痫前期组的胎盘组织染色范围和强度比较,差异无统计学意义(P>0.05)。结论:LIF对维持正常妊娠有一定的保护作用,LIF分泌或调控异常,影响了滋养细胞的浸润能力,导致病理妊娠,在胎盘组织中的低表达,参与了子痫前期发病机制。

Objective： To determine the expression of leukemia inhibitory factor（LIF） in preeclampsia placenta, and to assess the effects of LIF on the pathogenesis of pregnancy induced hypertension syndrome. Methods ： A streptavidin peroxidase conjugated semi -quantitative immunohistochemical assay was used to determine LIF protein expression in normal pregnancy group and preeclampsia groups（ mild preeclampsia group and serious preeclampsia group）. Results： LIF protein expression was observed in all cases and located mainly in the cytoplasm of trophoblast cells. The expression of LIF protein in preeclampsia groups obviously increased compared to the normal pregnancy group（ P 〈 0.01 ）. However, there was no significant difference in the expression of LIF between the two preeclampsia groups （P 〉 0.05）. Conclusion： LIF may play a role in the maintenance of normal pregnancy. Abnormality of LIF expression in placenta can affect the cytotrophoblastic invasion and placental vascular reconstruction and lead to pathological pregnancy. Pathogenesis of Preeclampsia may due to the reduction of LIF expression in placenta.