应用大孔树脂吸附分离技术制备地锦草总黄酮的研究

Study on Preparation of Euphorbia humifusa Total Flavonoids by the Adsorption and Separation of Macroporous Resins

目的研究大孔树脂DA201和D101富集地锦草总黄酮的工艺条件和参数。方法以总黄酮含量为指标,采用正交试验设计,考察DA201和D101富集地锦草总黄酮的工艺条件。用分光光度法测定总黄酮含量。结果DA201精制的最佳工艺为:90 mL浓度约0.65 mg.mL-1的溶液(pH 7)上柱,吸附流速2 BV.h-1,洗脱剂为40%乙醇(pH 7),洗脱流速2 BV.h-1。D101精制的最佳工艺为:100 mL浓度约0.65 mg.mL-1溶液(pH 7)上柱,吸附流速4 BV.h-1,洗脱剂为40%乙醇(pH 9),洗脱流速2 BV.h-1。通过DA201和D101精制工艺,平均吸附率分别为83.38%和88.57%,平均洗脱率分别为89.28%和87.21%,洗脱液干燥后的总固物中总黄酮平均含量分别为14.48%和20.18%,分别高于原上样液总固物黄酮含量(8.49%和8.71%)。结论DA201和D101对地锦草总黄酮都有良好吸附分离性能,而D101综合性能更好。

OBJECTIVE To study the optimal technological parameters of the enrichment process for total flavonoids of Euphorbia humifusa with macroporous resins DA201 and D101. METHODS The technological conditions of the DA201 and D101 enrichment process were studied by orthogonal experiment design with the content of total flavonoids from Euphorbia humifusa as indexes. Spectrophotome- try was used for the determination of total flavonoids. RESULTS The optimum technological conditions of the Dλ201 refining were： 90mL of the solution concentration at 0.65 mg · mL^ -1 （ pH 7 ）, the current velocity of adsorption 2BV · h ^-1, the eluting reagent 40% ethanol（ pH 7） ,the eluting velocity 2BV · h^ -1. The optimum technological conditions of the Dl01 refining were： 100mL of the solution concentration at 0.65 mg · mL^-1（ pH 7） ,the current velocity of adsorption 4BV · h^-1 ,the eluting reagent 40% ethanol（ pH 9） ,the eluting velocity 2BV · h^-1. By means of refining technology of the D101 or 101 , the average adsorbing rates of total flavonoids were 83.38% and 88.57% ,the average elutive rate of total flavonoids were 89.28% and 87.21%, respectively, the average content of total flavonoids were 14.48% and 20.18% respectively in the total solid of the ethanol eluate. The average content after D101 or D101 refining were higher than the ones （8.49% and 8.71% ） of original solution respectively. CONCLUSION The D101 and D101 type macroporous resins had the favourable performance for the adsorbing and separating the total flavonoids from Euphorbia humifusa. D101 showed better comprehensive property.