快速柱前衍生-高效液相色谱法测定丙戊酸血药浓度

Determination of Serum Valproic Acid Concentration by HPLC after Precolumn Derivatization

目的 建立一种简便可行的快速柱前衍生-高效液相色谱法（HPLC）测定丙戊酸血药浓度.方法 患者血清经正己烷提取后在C18柱上分析,流动相为甲醇-水（75∶25）,柱温30 ℃,检测波长254 nm,流速1 mL·min^-1.结果 丙戊酸在12.5～400 μg·mL^-1范围内线性关系良好,日内和日间RSD均小于10%（n=5）.结论 本法简便、稳定,用于丙戊酸的血药浓度监测,效果良好.

OBJECTIVE To Determine serum valproic acid concentration by HPLC. METHODS Serum sample was extracted with n-Hexane and analyzed on a Cls column, The mobile phase consisted of methanol ：deionized water（ 75：25 ）, at a temperature of 30 ℃. The compound was quantified by UV absorbance at a wavelength of 254 nm with a flow rat of 1 mL · min ^-1. RESULTS The calibration curve of valproic acid in serum was linear in the range of 12.5 -400 μg · mL^-1. The overall precision expressed as RSD（ n = 5 ） was less than 10% for intra-day and inter-day. CONCLUSION This method was shown to be simple, convenient, reliable and suitable for monitoring the blood concentrations of valproic acid.