摘要
目的建立青霉素V钾分散片含量的HPLC测定方法.方法色谱柱:Hypersil BDS-C18(4.6 mm×200mm,5μm),流动相为水-乙腈-冰醋酸(65∶35∶1),检测波长为268nm.结果青霉素V进样量在10μg^50μg的范围内与峰面积呈良好的线性关系(r=0.9998).平均回收率为99.7%,RSD=0.70%.结论该方法简便,快速,重复性好.
OBJECTIVE To establish a method for determination of phenoxymethyl penicillin potassium dispersible tablets by HPLC. METHODS Column： Hypersil BDS-C18 （4. 6 mm × 200mm, 5μm ）. Mobile phase： Water-Acetonitrile-acetic acid （65 ： 35 ： 1）. Detection wavelength：268nm. RESULTS A linear relationship was good from 10μg to 50μg（ r =0. 9998） . The average recovery was 99. 7% with RSD =0. 70%. CONCLUSION The method is simple,reproducible and can be used for the quality control.
出处
《齐鲁药事》
2007年第8期470-471,共2页
qilu pharmaceutical affairs
关键词
高效液相色谱
青霉素V钾分散片
含量测定
HPLC
phenoxymethyl penicillin potassium dispersible tablet
Determination
