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Echistatin对人增生性瘢痕成纤维细胞的作用研究 被引量：2

Research of echistatin effect on human hypertrophic scar-derived fibroblasts
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摘要 目的研究Echistatin对人增生性瘢痕成纤维细胞增殖、凋亡及Ⅰ、Ⅲ型前胶原mRNA表达的影响。方法体外培养人增生性瘢痕成纤维细胞,设Echistatin处理组和对照组,MTT法、流式技术及RT-PCR法检测人增生性瘢痕成纤维细胞增殖、凋亡及Ⅰ、Ⅲ型前胶原mRNA的表达。结果Echistatin可明显抑制人增生性瘢痕成纤维细胞增殖,促进凋亡,降低Ⅰ、Ⅲ型前胶原mRNA的表达。结论Echistatin可作为防治增生性瘢痕的筛选药物。 [Objective] To investigate the effect of echistatin on proliferation, apoptosis, and type Ⅰ and type Ⅲprocollagen mRNA expression in human hypertrophic scar-derived fibroblasts. [Methods] After treated by eehistatin, proliferation, apoptosis, type Ⅰ/type Ⅲ procollagen mRNA expression in cultured fibroblasts from human hypertrophic sear were determined by MTT method, Flow eytometry , and RT-PCR respectively. [Results] Eehistatin treatment resulted in significant inhibition of proliferation, type Ⅰ/type Ⅲ proeollagen mRNA expression, and marked apoptosis in cultured hypertrophic scar-derived fibroblasts. [Conclusion] Eehistatin may be chosen as a drug to prevent and cure hypertrophic scar.
作者 范鹏举 李珍 黄晓元
机构地区 中南大学湘雅医院烧伤整形科 中南大学湘雅二院ICU
出处 《中国医学工程》 2007年第6期469-472,共4页 China Medical Engineering
关键词 ECHISTATIN 成纤维细胞 瘢痕 eehistatin fibroblast scar
分类号 R392.28 [医药卫生—免疫学]
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共引文献34

同被引文献18

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中国医学工程
2007年 第6期

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