CXJZ95-198菌株β-甘露聚糖酶的纯化条件研究

Study on Purification of β-Mannanase from CXJZ95-198 Strain

研究通过超滤、硫酸铵沉淀、丙酮沉淀及凝胶层析等纯化步骤,将CXJZ95-198菌株所分泌的β-甘露聚糖酶进行了纯化,其纯化倍数为12.05,收率为33.50%。通过SDS-聚丙烯酰胺电泳得到单一条带蛋白,分子量为52.29 kD。

The β-mannanase from CXJZ95-198 sirain was purified by ultraflltrafion, ammonium sul-fate precipitate, acetone precipitate and gel chromatography. The final yield is 33.50% and purification folds are 12.05.The single protein band was obtained by SDS-PAGE and the molecular weight of the β-Man-nanase is 52.29kD.