期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰及其应用 被引量：1

RNA Interference and Its Application
下载PDF
导出
摘要 RNA干扰(RNA interference,RNAi)现象存在的广泛性远远超过人们的预期。研究表明RNAi具有特异性和高效性,已经成为研究基因功能的重要工具。文章阐明了RNAi的作用机制及其在基因治疗、药物筛选、研究信号传导通路等方面的重要应用价值,并对RNAi技术及应用研究进行了展望。 RNA interference （RNAi） is discovered in many more organisms than what researchers expect. Owing to its efficiency and specificity, RNAi has become an important tool in the research of genie functions. This paper explores RNAi＇s function mechanism and its important implications in genie therapy, medicine screening and the research of signal transmission pathway, etc. It also predicts the future of RNAi technology and the research of its application.
作者 马红樱 吴伟
机构地区 黄石理工学院医学院
出处 《黄石理工学院学报》 2007年第4期68-70,共3页 Journal of Huangshi Institute of Technology
关键词 RNA干扰 转录后基因沉默 小干扰RNA RNA诱导的沉默复合物 RNA interference posttranscriptional gene silencing small interfering RNA RNA- induced silencing complex
分类号 Q752 [生物学—分子生物学]
  • 相关文献

参考文献17

  • 1Napoli C,Lemieux C,Jorgensen R.Introduction of achalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans[J].Plant Cell,1990(2):279-289.
  • 2Guo S,Kemphues KJ.Par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinase that is asym-metrically distributed[J].Cell,1995,81:611-620.
  • 3Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabdits elegans[J].Nature,1998,391:806-811.
  • 4Zamore PD,Tuschl T,Sharp PA,et al.RNAi:double-stranded RNA directs the ATP-dependnd cleavage of mRNA at 21to23 nucleotide intervals[J].Cell,2000,101:25-33.
  • 5Hannon GJ.RNA interference[J].Nature,2002,418(6894):244-251.
  • 6袁婺洲,Bodmer R,朱传炳,王跃群,李永清,吴秀山.利用RNAi技术研究果蝇心脏发育基因的功能[J].Acta Genetica Sinica,2002,29(1):34-38. 被引量：30
  • 7Harborth J,Elbashir S M,Bechert K,et al.Identification of essential genes in cultured mammalian cells using small interfering RNAs[J].J Cell Science,2001,114(24):4557.
  • 8Wilda M,Fuchs U,Wossmann W,et al.Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference(RNAi)[J].Oncogene,2002,21(37):5716-5724.
  • 9Gitlin L,Karelsky S,Andino R.Short interfering RNA confers intracellular antiviral immunity in human cells[J].Nature,2002,418:430-434.
  • 10Novina C D,MurrayMF,Dykxhoorn DM,et al.siRNA directed inhi-bition of HIV-1infection[J].Nat Med,2002,8(7):681-686.

二级参考文献34

  • 1[12]Wu X, Park M, Golden K et al. The wingless signaling pathway is directly involved in Drosophila development. Developmental Biology, 1996, 177:104～116.
  • 2[13]Kennerdell J R, Carthew R W. Use of deRNA-Mediated Genetic Interference to Demonstrate that frizzled and frizzled 2 Act in the wingless Pathway. Cell, 1998, 96:1017～1026.
  • 3[14]Brand A H, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development, 1993, 118:401～415.
  • 4[15]Rorth P, Szabo K, Bailey A et al. Systematic gain-of-function genetic in Drosophila. Development, 1998, 125:1049～1057.
  • 5[1]Fire A, Xu A Q, Montgomery M K, Kostas S A, Driver S E, Mello C C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans . Nature, 1998, 391:806～811.
  • 6[2]Fire A. RNA-triggered gene silencing. TIG, 1999, 15(9):358～363.
  • 7[3]Hammond S M, Bernstein E, Beach D, Hannon G J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 2000, 404: 293～296.
  • 8[4]Svoboda P, Stein P, Hayashi H, Schultz R M. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development, 2000, 127:4147～4156.
  • 9[5]Kennerdell J R, Carthew R W. Heritable gene silencing in Drosophila using double-stranded RNA. Natural Biotechnology, 2000,17:896～898.
  • 10[6]Misquitta L, Paterson B M. Targeted disruption of gene function in Drosophila by RNA interference (RNAi): Arole for nautilus in embryonic somatic muscles formation. Proc. Natl. Acad. Sci. USA, 1999, 96:1451～1456.

共引文献39

同被引文献9

  • 1张中华,侯永泰.siRNA制备技术的研究进展[J].生命科学,2004,16(4):231-235. 被引量：12
  • 2阚学峰,高英堂,杜智.SiRNA四种制备技术的介绍[J].国外医学（临床生物化学与检验学分册）,2004,25(4):342-344. 被引量：4
  • 3崔照琼,王旭东.RNA干扰技术研究进展及其应用[J].中国畜牧兽医,2005,32(3):27-31. 被引量：6
  • 4Julie C, Sheng D. Generation of RNAi Libraries for High- Throughput Screens[J]. Journal of Biomedicine and Bio technology, 2006,45716 : 1.
  • 5Wang S, Shi Z, Liu W, et al. Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing[J]. BMCBiotechnol, 2006,6:50.
  • 6Chen M, Du Q, Zhang HY, et al. Vector-based siRNA delivery strategies for high through put screening of novel target genes [J]. Journal of RNAi and Gene Silencing, 2005, 1 (1) : 5.
  • 7Daniel C, Doris L, Volker W. Short interfering RNA (siRNA): tool or therapeutic [J]. Clinical Science, 2006, 110:47.
  • 8Berns K, Hijmans EM, Mullenders J, et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway[J]. Nature, 2004,428: 431.
  • 9Kim D-H, Behlke MA, Rose SD, et al. dsRNA Dicer substrates enhance RNAi potency and efficaey[J]. Nature Biotechnology, 2005,23(2): 222.

引证文献1

二级引证文献1

黄石理工学院学报
2007年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部