壮肝逐瘀煎对肝纤维化大鼠TGF-β1的影响

Effects of Zhuanggan Zhuyu Decoction on Expression of TGF-β_1 in Rats with Hepatic Fibrosis

目的:探讨壮肝逐瘀煎在抗肝纤维化中的治疗作用机理。方法:Wistar大鼠120只,随机取16只为正常对照组(A),其余采用四氯化碳复合因素诱导大鼠肝纤维化,造模四周证实肝纤维化形成后随机分为病理模型组(B),壮肝逐瘀煎大、中、小剂量组(C、D、E),秋水仙碱组(F),大黄虫丸组(G),分别灌胃给药,A、B组予等容积生理盐水灌胃。于治疗12周后每组各取8只大鼠处死并取血清及肝组织,HE染色观察肝纤维化的形成,酶联免疫吸附实验法检测血清中TGF-β1因子的表达差异。结果:与病理模型组比较,C、D、E、F、G组肝纤维化程度均明显减轻(P<0.05或P<0.01),其中D组与F组比较有显著性差异(P<0.05);各治疗组血清中TGF-β1水平显著下降(P<0.01),C、D组降低血清TGF-β1水平均优于F、G组(P均<0.01)。结论:壮肝逐瘀煎能有效地改善实验大鼠肝纤维化程度,其机理可能与降低肝纤维化大鼠血清中TGF-β1水平有关。

objective： To explore the curative mechanisms of Zhuanggan Zhuyu Decoction in treating hepatic fibrosis. Methods： Among 120 Wister rats, 16 were randomly selected for the normal control group （A）, the rest were made into the hepatic fibrosis animal models by using Carbon tetmchloride （CCL）, and then the rat models were randomly divided into six groups： the model control group （B）, the Zhuartggan Zhu3u Decoction groups [the high - dose group （C）, the intermediate - dose group （D） and the low - dose group （E） ], the colchicines group （F） and the Dahuang Zhechong Bolus group （G）. The treatment was performed in the form of introgastric administration. The rats were killed at the end of the twelfth week in order to collect serum and perform liver biopsy. The formation of hepatic fibrosis was observed by using HE stain and the expression of TGF- β1 was detected by ELISA. Results： Compared with the model control group （B）, group C, D and E all showed significant reversion of hepatic fibrosis upon pathological examination （P 〈 0.05） and had significant decrease of the level of TGF- β1（p 〈 0.05）. Conclusions： Zhuanggan Zhuyu Decoction has a protective effect on liver injury and can inhibit CCL4- induced hepatic fibrosis in rats. These mechanisms may be associated with its effects of down - regulating the level of TGF -β1.