Mb-Collagen薄膜电极对O_2,H_2O_2的电化学催化

Electrocatalysis of O_2 and H_2O_2 at Myoglobin-Collagen Film Electrodes

用循环伏安法研究了肌红蛋白(Mb)在胶原蛋白(Collagen)薄膜电极的电化学行为.研究表明,胶原蛋白具有一定的生物适应性,提供了适合于肌红蛋白与石墨电极之间进行直接电子转移的微环境.Mb-Collagen薄膜的循环伏安峰出现在-0.35 V(vs.SCE)左右,为Mb Fe()/Fe()电对的特征峰.H2O2,O2是许多氧化酶催化反应的产物,具有一定的生物意义.Mb-Collagen薄膜可用于对H2O2和O2的电化学催化还原,使它们的还原过电位显著降低,显示了该体系在构筑新型的基于蛋白质直接电化学的生物传感器方面具有潜在的应用价值.

The electrochemical properties of myoglobin（Mb） in collagen films electrodes were investigated with cyclic voltammetry. As their biocompatibility, Collagen offers a suitable microenvironment for myoglobin to transfer electron directly with the underlying electrodes. Cyclic voltammetry of Mb-Collagen films show a pair of well-defined and nearly reversible peaks at about -0.35 V （vs. SCE）, characteristic of Mb Fe （ Ⅲ ）/Fe （ Ⅱ ） redox couple. Oxygen and hydrogen peroxide are product of electrocatalyze reduction for oxidase, and have the biological significance. They can be used to electrocatalyze reduction of oxygen and hydrogen peroxide with significant decrease of the reduction overpotential. This indicates potential value of the films for the fabrication of a new type of biosensors based on the direct electrochemistry of redox proteins.