刺五加叶中成分的定量标准研究

Study on quantitative analysis standard of constituents in the leaves of Acantopanax senticosus (Rupr.et Maxim) Harms

采用薄层扫描法测定了刺五加叶中皂苷配基之一齐墩果酸，对刺五加叶中皂苷的水解条件、皂甙配基的薄层层析条件及显色条件进行了研究。确定了用５％Ｈ２ＳＯ４乙醇水（１∶１）溶液，回流１０ｈ，在予制高效硅胶薄层层析板，以苯—丙酮（３６∶１３）为展开剂展开，喷１０％Ｈ２ＳＯ４水溶液，１０５℃烘烤１５ｍｉｎ显色条件，测定了刺五加叶总皂苷中齐墩果酸的含量。本方法的平均回收率９８％，相对标准偏差为３．０％。

Oleanolic acid,one of the aglycones of the saponins in the leaves of Acantopanax senticosus (Rupr.et Maxim) Harms was determined using a TLC scan method.Influential factors of the saponin hydrolysis and visualization were examined.The key experimental conditions were as follows: saponin hydrolysis at 100℃ with 5% H 2SO 4 ethanol water (1∶1) solution for 10 h,TLC separation on a precoated silica gel plate with benzene acetone (36∶13) as a developer,visualization at 105℃ for 15 min after spraying 10% H 2SO 4.The average rate of recovery and RSD of the method were 98% and 3 0%,respectively.