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固相合成血管紧张素Ⅱ及其反相高效液相色谱纯化与分析 被引量：1

Purification and analysis of solid phase synthetic angiotensin Ⅱ by RP-HPLC
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摘要 目的用固相方法合成血管紧张素Ⅱ,建立其纯化和分析条件。方法以Fmoc-Phe-Wang树脂为固相载体,采取Fmoc/tBu正交保护固相合成策略,粗品的纯化采用制备型RP-HPLC。结果经过分析型RP-HPLC分析最后纯品的纯度>98%,并用电喷雾接口质谱分析证实了分子质量的正确性。结论用Fmoc固相法合成血管紧张素Ⅱ快速简便、操作方便而且回收率很高,最后通过RP-HPLC纯化,可以得到纯度很高的血管紧张素Ⅱ。 Objective To synthesize angiotensin Ⅱ by solid phase method, and to establish the conditions of purification and analysis. Methods Angiotensin Ⅱ was synthesized by Fmoc/tBu orthogonal protection strategy on Fmoc-Phe-Wang resin. The crude peptide was purified using preparative RP-HPLC. Results The purity of the final product confirmed by analytical RP- HPLC was more than 98%. Electrospray interface mass spectrometric analysis showed the right molecular mass of the synthetic peptid.Conclusion The Fmoc solid phase method is simple and easy in the synthesis of angiotensin Ⅱand the yield of the final product is high. The pure angiotensin Ⅱthrough the preparation of RP-HPLC has been obtained at last.
作者 浦迎秋 闫琰 黄艳
机构地区 南京大学医学院附属口腔医院 南京医科大学附属脑科医院 香港澳美制药厂
出处 《中南药学》 CAS 2007年第4期308-311,共4页 Central South Pharmacy
关键词 血管紧张素Ⅱ 多肽 固相合成 反相高效液相色谱 angiotensin Ⅱ peptide solid phase synthesis RP-HPLC
分类号 R917 [医药卫生—药物分析学] TQ460 [化学工程—制药化工]
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参考文献8

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二级参考文献26

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中南药学
2007年 第4期

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