婴儿痉挛症血清NSE、S100B水平动态变化

Study on ambulatory changes of neuron-specific enolase and S100B level in infants with infantile spasm

目的探讨婴儿痉挛症(infantile spasm,IS)患儿痉挛发作期间及发作控制后神经元特异性烯醇化酶(neuron specificenolase,NSE)、S100B的表达水平及其与脑损伤的关系。方法采用ELISA方法定量检测30例IS患儿发作期间、发作控制48h内及1周后血清NSE、S100B浓度;30名同年龄组健康体检儿设为对照。实验结果进行统计学处理。结果IS发作期和发作控制48h内血清NSE水平有显著差异(P<0.05),但二者均显著高于发作控制1周后水平(P<0.01)。S100B血清浓度在治疗前与发作控制48h内无显著差异(P>0.05),二者也显著高于发作控制1周后(P<0.01)。观察组治疗前和发作控制48h内NSE、S100B血清浓度显著高于对照组(P<0.01);IS发作控制1周后血清NSE、S100B浓度和对照组无明显差异(P>0.05)。结论IS患儿痉挛发作期间和发作控制短期内NSE、S100B水平持续增加,可作为判断IS早期脑损伤的生化标记并可能影响IS患儿的远期预后。

Objective To explore ambulatory changes of serum neuron-specific enolase and S100B level in infants with infantile spasm and to evaluate the relationships among NSE, S100B levels and cerebral injury. Methods Serum NSE, S100B levels were measured using an enzyme-linked immunosorbent assay （ELISA） in 30 infants with infantile spasm （IS group）. The blood samples were collected before the patients were treated and controlled 48 hours, and after controlled one week, respectively. IS patients were compared with 30 healthy controlls （CON group） matched by age and sex. Results Significantly high levels of NSE,S100B were observed in infants with infantile spasm before treated and controlled 48 hours than after controlled one week in the patients and CON group （P〈0.01）. No difference was observed in NSE,S100B levels between IS patients after controlled one week and CON group （P〉0. 05）. Serum NSE levels were significantly difference in IS patients before treated and controlled 48 hours （P〈0. 05） Conclusion Serum concentration and kinetics of NSE,SIOOB provide the clinical assessment of the primary brain damage and have a predictive value for outcome in infants with infantile spasm.