期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

上游刺激因子1在小鼠牙齿发育过程中表达的原位杂交研究

In situ hybridization research of USF1 mRNA expression in mouse tooth development
下载PDF
导出
摘要 目的:检测小鼠牙齿发育中上游刺激因子1mRNA的表达及时空分布模式。方法:制备E13,16,19,P1,5,8,11,21d小鼠第一磨牙石蜡切片,用我们前期实验制备的USF1cDNA探针,原位杂交方法检测USF1mRNA的表达和分布。结果:原位杂交染色从钟状晚期小鼠牙胚中开始检测到阳性信号,持续至P11d,阳性信号主要定位于成牙本质细胞与成釉细胞;而蕾状期,帽状期,钟状期牙胚中未检测到阳性信号,并且在牙齿萌出后(P21d),牙齿中USF1mRNA阳性信号再次消失。结论:牙胚中有USF1mRNA表达,主要位于分泌期的成牙本质细胞和成釉细胞,表达模式具有显著的时空特异性。 AIM： To detect the temporo - spatial expression of USF1 mRNA during mouse tooth development. METHODS： Paraffin sections of first molar tooth germ in El3, 16, 19, P1, 5, 8, 11, 21d mouse were prepared respectively and in situ hybridization was carried out with the specific USF1 cDNA probe prepared in our previous researches. RESULTS： Positive staining for USF1 mRNA in mouse tooth germ began from P1 d evidently, and extended to P1 ld, which was mainly localized in the secreting ameloblasts and odontoblasts, but no staining in bud, cap and early bell stages of tooth germ. Moreover, on P21d after tooth eruption, USF1 mRNA became negative again. CONCLUSlON： USF1 mRNA is expressed in tooth germ, which mainly localizes in the secreting ameloblasts and odontoblasts, and its expression was quite dynamic during mouse tooth development.
作者 吴礼安 文玲英 杨富生 王小竞
机构地区 第四军医大学口腔医学院
出处 《牙体牙髓牙周病学杂志》 CAS 2007年第8期436-438,共3页 Chinese Journal of Conservative Dentistry
关键词 上游刺激因子1 小鼠牙胚 MRNA 表达 原位杂交 USF1 mouse tooth germ mRNA expression in-situ hybridization
分类号 R780.2 [医药卫生—口腔医学]
  • 相关文献

参考文献9

  • 1吴礼安,文玲英,杨富生,樊淑梅.牙胚中上游刺激因子部分序列的cDNA克隆[J].牙体牙髓牙周病学杂志,2002,12(11):588-590. 被引量：4
  • 2吴礼安,文玲英,杨富生,刘勇.成牙本质细胞中上游刺激因子1表达的原位杂交研究[J].上海口腔医学,2003,12(2):120-122. 被引量：2
  • 3Khan M,Seppala M,Zoupa M,et al.Hedgehog pathway gene expression during early development of the molar tooth root in the mouse[J].Gene Expr Patterns,2007,7(3):239-243
  • 4Sorkar L,Cobourne M,Naylor S,et al.Wnt/Shh interactions regulate ectodermal boundary formation during mammalian tooth development[J].Proc Natl Acad Sci,2000,97(9):4520-4524
  • 5Pereira TV,Salzano FM,Mostowska A,et al.Natural selection and molecular evolution in primate PAX9 gene,a major determinant of tooth development[J].Proc Natl Acad Sci,2006,103(15):5676-5681
  • 6Wang XP,Aberg T,James MJ,et al.Cbfa1 inhibits Shh signaling in the lower but not upper molars of mouse embryos and prevents the budding of putative successional teeth[J].J Dent Res,2005,84(2):138-143
  • 7Corre S,Galibert MD.Upstream stimulating factors:highly versatile stress-responsive transcription factors[J].Pigment.Cell Res,2005,18(5):337-348
  • 8Asaumi S,Takemoto M,Yokote K,et al.Identification and characterization of high glucose and glucosamine responsive element in the rat osteopontin promoter[J].J Diabetes Complications,2003,17 (1):34-38
  • 9Zhu Y,Casado M,Vaulont S,et al.Role of upstream stimulatory factors in regulation of renal transforming growth factor-beta1[J].Diabetes,2005,54(7):1976-1984

二级参考文献19

  • 1Sawadogo M,Roeder RG.Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region [J]. Cell, 1985, 43(1): 165-175.
  • 2Henrion A A, Vaulont S, Raymondjean M, et al. Mouse USF1 gene cloning: comparative organization within the c-myc gene family[J].Mammalian Genome, 1996, 7(11): 803-809.
  • 3Ebara S, Kawasaki S, Nakamura I, et al. Transcriptional regulation of the mBMP-4 gene through an E-box in the 5' -flanking promoter region involving USF [J]. Biochem Biophys Res Commun,1997, 240(1): 136-141.
  • 4Sirito M, Lin Q,Deng JM, et al. Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice [J]. Proc Natl Acad Sci, 1998, 95(7): 3758-3763.
  • 5Narayanan K, Srinivas R, Ramachandran A, et al. Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1 [J]. Proc Natl Acad Sci,2001, 98(8): 4516-4521.
  • 6Sun ZL, Fang DN, Wu XY, et al. Expression of dentin sialoprotein(DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23 [J]. Connect Tissue Res, 1998, 37(3-4): 251-261.
  • 7Bronckers AL, Engelse MA, Cavender A, et al. Cell-specific patterns of Cbfal mRNA and protein expression in postnatal murine dental tissues[J]. Mech Dev, 2001, 101(1-2): 255-258.
  • 8Viollet B, Lefrancois-Martinez AM, Henrion A, et al. Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms J. J Biol Chem,1996,271(3): 1405
  • 9Qyang Y, Luo X, Lu T, et al. Cell-type-dependent activity of the ubiquitous transcription factor USF in cellular proliferation and transcriptional activationJ. Mol Cell Biol,1999,19(2): 1508
  • 10Sirito M, Lin Q, Deng JM, et al. Overlapping roles and asymmetrical cross-regulation of the USF proteins in miceJ. Proc Natl Acad Sci USA,1998, 95(7):3758

共引文献4

牙体牙髓牙周病学杂志
2007年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部