紫杉醇对HEC-1-B细胞凋亡诱导作用的实验研究

Induction by taxol to apoptosis of HEC-1-B cells

目的:探讨紫杉醇对体外培养的人子宫内膜腺癌细胞(HEC-1-B)细胞凋亡的诱导作用.方法:体外培养HEC-1-B细胞,用浓度2,4,8,16,20nmol/L的紫杉醇作用细胞12h,24h,48h后,提取细胞DNA片段进行琼脂糖凝胶电泳;消化HEC-1-B细胞并经碘化丙锭(PI)染色后,流式细胞仪(FCM)对细胞进行细胞周期分析;电子显微镜对紫杉醇作用后的HEC-1-B细胞进行形态学分析.结果:体外培养的HEC-1-B细胞经不同浓度的紫杉醇处理后,细胞生长明显受到抑制;于8nmol/L紫杉醇作用48h后可观察到细胞凋亡特异的DNA梯状电泳;流式细胞仪分析证实,细胞凋亡率达19.7%;且电镜下可观察到典型的凋亡早期形态学改变.结论:人子宫内膜腺癌细胞(HEC-1-B)细胞对紫杉醇具有药物敏感性,紫杉醇对HEC-1-B细胞的杀伤作用,是通过诱导细胞凋亡途径实现的.

AIM： To investigate the inhibition of proliferation and the induction of apoptosis in in vitro cultured human endome- trial adenocarcinoma cell HEC-1-B by taxol. METHODS： Human endometrial adenocarcinoma HEC-1-B cells were cultured in vitro and treated with 2, 4, 8, 16, 20 nmol/L taxol for 12, 24, 48 h respectively. Isolated DNA fragments from HEC-1-B cells were analyzed by agrose gel electrophoresis. Apoptotic index in HEC-1-B cells stained with PI was detected with flow cytometer （FCM） assay. Electron microscopy was used to observe the morphological changes of HEC-1-B cells after treated with taxol. RESUILTS ： After treated with 8 nmol/L taxol for 48 h, DNA ladder tormation was observed in HEC-1-B cells. Typical apoptotic appearance was seen under an electron microscope. FCM assay revealed that the apoptotic rate was 19.7%. CONCLUSION： Taxol inhibits the proliferation and induces the apoptosis of human endometrial adenocareinoma cell HEC-1-B.