建立大鼠肝动脉插管途径的方法学研究

Methodological evaluation for catheterization of hepatic artery in rat

目的研究并完善直接经胃十二指肠动脉向大鼠肝脏灌注药物或细胞悬液的方法。方法直视下经大鼠胃十二指肠动脉行肝动脉插管并注入药物或细胞悬液。结果SD大鼠52只,47只存活且术后恢复良好,4只死亡,1只插管未能成功。死亡原因可能为血管受损、肝脏损伤、麻醉意外和术后感染等。死亡原因在操作技术熟练以及加强术后护理后可以避免。结论大鼠胃十二指肠动脉插管进行肝动脉灌注的方法简单、容易掌握、成功率高,适用于研究药物或细胞悬液经肝动脉灌注对肝脏的作用。

Objective To search tor a modified route tor catheterization of hepatic artery via gastroduodenal artery approach. Methods The drug or cell suspension was infused into hepatic artery in rat via gastroduodenal artery route by opening operation. Results Fifty two rats were enrolled with 47 alive and recovered from operation quickly, but 4 of them died and the other one was failure clue to having too small artery to be catheterized. The mortality might possibly be vascular injury, liver damage, accident in anesthesia or infection after operation and skillful manipulation is necessary for prevention. Conclusion The way of hepatic artery infusion in rat via gastroduodenal artery route is simple, easy to be mastered, and it is suitable to study the effect of drug or cell suspension on liver. （J Intervent Radiol, 2007, 16： 264-265）