包头蒙古族Y染色体的遗传多态性研究

Genetic polymorphism of Y-chromosome STR in Mongolia Ethnic Group of Baotou City

目的调查包头蒙古族Y染色体上10个STR基因座及单倍型的遗传多态性。方法应用PCR、变性聚丙烯酰胺凝胶电泳结合银染显色分型技术,检测99例无关系蒙古族男性血样。结果在DYS392、DYS438、DYS439、DYS456、DYS459、DYS460、DYS461、DYS462、DYS389Ⅰ、DYS389Ⅱ10个基因座中共检出43个等位基因,各基因座DP值分布在0.458(DYS462)至0.727(DYS389Ⅰ、DYS389Ⅱ)之间,除了DYS462(0.458)以外,其余基因座DP值均大于0.5。由10个基因座组成的YH系统单倍型有98种,DP值为0.98。结论上述10个Y-STR基因座的单倍型具有较高的遗传多态性。

To study the genetic polymorphism of 10 Y-chromosome STR loci by the allelic frequencies and haplotype frequencies in a Mongolia population sample of Baotou city. Methods We used PCR and polyacrylamidedel electrophoresis （PAGE） following silver staining to analyze the blood sample from the 99 unrelated Mongolia male individuals. Results The allele diversity values for each locus ranged from 0.458 （DYS462） to 0. 727（DYS389I, DYS389]D and gene diversity was above 0.5 except DYS462 （0. 458） in DYS392, DYS438, DYS439, DYS456, DYS459, DYS460, DYS461, DYS462, DYS389I and DYS389Ⅱ loci. Furthermore, 98 haplotypes were found from 10 loci and haplotype diversity was 0.98. Conclusion The haplotypes of the 10 Y-STR loci have the higher genetic polymorphism.