香附对未孕大鼠离体子宫肌收缩的影响

Effect of nutgrass galingale rhizome decotion on contractile activity of uterine smooth muscle strips of non-pregnant rats in vitro

目的探讨香附对未孕大鼠离体子宫平滑肌条运动的影响及其机制。方法选用Wistar雌性未孕大鼠60只,体重250-300 g,用Biolap410生物机能仪记录正常子宫平滑肌肌条收缩运动,并分别用苯海拉明(2×10-6mol/L)、酚妥拉明(2×10-6mol/L)、异搏定(2×10-7mol/L)、吲哚美辛(2×10-5mol/L)作用于肌条5 min后,加入香附水煎醇沉液(以下简称香附)以及单用香附,观察对离体子宫平滑肌运动的影响并分析作用机制。结果香附减弱未孕大鼠离体子宫平滑肌的收缩运动,收缩波的频率减慢,振幅减小,持续时间缩短。结论香附减弱未孕大鼠离体子宫平滑肌的收缩运动,该作用可能是通过前列腺素的合成与释放,与L-型钙通道、H1受体、α受体无关。

To study the effects of nutgrass galingale rhizome decotion on contractile activity of uterine smooth muscle strips of non-pregnant rats. Methods We selected 60 Wistar non-pregnant rats of weight 250-300 g to record the effects of nutgrass galingale rhizome decotion （1.0 g/L, 2.0 g/L, 4.0 g/L, 8.0 g/L） on contractile activity of uterine smooth muscle strips of non-pregnant rats with Biolap 410 Biological System. Four antagonists, diphenhydramine（2X 10 6 mol/L）, phentolamine （2 X 10 6 mol/L ）, verapamil （2 X 10-7 mol/L）, and indomethacin （2 X 10-s mol/L） were used to study their mechanisms respectively. Results Water extract of nutgrass galingale rhizome （4 X 10 3 kg/L） could significantly weaken uterine contractile activity of non-pregnant rats, lessen the frequencies, shorten the duration and lower the amplitude of contractile activity. Conclusion The effect of nutgrass galingale rhizome on contractile activity of uterine smooth muscle strips in rats may be mainly associated with prostaglandin synthese or release nothing with L-voltage-dependant calcium channels, Hi receptor or a receptor.