HLA-I类基因多态性与白血病易感性的关联性研究

Correlation between gene HLA-class I polymorphism and susceptibility to leukemia

目的探讨白血病易感性与HLA-I类基因多态性之间的关联性,寻找白血病的易感基因。方法采用序列特异性寡核苷酸探针杂交技术(PCR-SSO)对65例白血病患者和48例正常对照组健康者进行HLA-A、B基因分型。结果在等位基因HLA-A、B中,白血病患者的HLA-A01、B38基因的基因频率高于正常对照组(P<0.05),而HLA-A11基因的基因频率明显下降(P<0.05)。结论基因A01、B38对白血病有遗传易感作用,而基因A11对白血病有遗传拮抗作用。

To explore the correlation between gene HLA-class I polymorphism and susceptibility to leukemia in Chinese Gansu Han people and search for the genes susceptible to leukemia. Methods HLA-A and B alleles polymorphism in 65 patients with leukemia and 48 normal subjects were determined by PCR with sequence specific oligucleotide probe （PCR-SSO）. Results The allele frequencies of HLA-A01 and B38 were increased （P〈0.05）, while the allele frequencies of HLA-A11 were decreased in patients with leukemia （P 〈 0. 05）. Oonclusion HLA-A01 and B38 alleles seem to contribute to the genetic susceptibility to leukemia, while HLA-A11 seems to contribute to its genetic resistance.