苦参碱对U937细胞及人脐静脉血管内皮细胞增殖的影响

Effect of matrine on U937 cells and human umbilical vein endothelial cells proliferation

目的：观察苦参碱（Matrine，Mat）对U937细胞及人脐静脉血管内皮细胞（HUVECs）增殖的影响。方法：采用体外培养技术，通过细胞形态、MTT实验、细胞周期测定观察Mat对U937细胞及HUVECs增殖的影响。结果：Mat（0．2～0．5mg／mL）作用24h后对U937细胞均有增殖抑制作用（P〈0．05或P〈0．01），在该浓度范围内呈剂量和时间依赖性，其作用U937细胞48h的半数抑制浓度（IC50）约为0．4mg／mL，有效作用时间为1d；Mat（0．1～0．5mg／mL）作用24、48、72h后对HUVECs增殖无明显抑制作用，亦无剂量和时间依赖性（P〉0．05）；Mat（0．2～0．5mg／mL）作用U937细胞48h后，S期细胞比例增加，细胞发生S期阻滞（P〈0．05或P〈0．01）；Mat（0．1～0．5mg／mL）作用HUVECs 48h后，对细胞周期无明显影响（P〉0．05）。结论：一定浓度Mat对U937细胞具有增殖抑制作用，呈时间-剂量依赖关系。Mat在一定浓度和时间下不能抑制HUVECs增殖，对其细胞周期亦无明显影响。

AIM： To observe the proliferative effects of matrine on U937 cells and human umbilical vein endothelial cells. METHODS： The effects of matrine on U937 cells and human umbilical vein endothelial cells proliferation were observed by morphology, MTT and cell cycle detection. RESULTS： U937 cells treated with matrine at the concentration of 0.2 mg/mL to 0.5 mg/mL for 24 h were inhibited proliferation in a time and dose-dependent manner detected by MTT （P 〈 0.05 or P 〈 0.01） and increased at the S stage （ P 〈 0.05 or P 〈0.01）. The half-inhibition concentration （ IC50 ） was 0. 4 mg/mL in U937 ceils compared with human umbilical vein endothelial cells （P 〉 0.05）. HUVECs treated with matrine at the concentration of 0.1 to 0.5 mg/mL for 48 h showed no effect on cell cycle （ P 〉 0.05）. CONCLUSION： Matrine has an inhibited effect on U937 ceils proliferation in a time and dose-dependent manner.