摘要
目的:建立测定人血浆中瑞舒伐他汀钙的液相色谱.串联质谱法,考察瑞舒伐他汀钙在中国健康志愿者体内的药代动力学。方法:含有瑞舒伐他汀钙和氟伐他汀钠的血浆样品经液-液提取后,进行色谱分析,在三重四极杆串联质谱仪上,以多反应离子监测方式进行定量分析。结果:瑞舒伐他汀钙的定量下限为0.03ng/mL,线形范围为0.03~60ng/mL,精密度与准确度符合生物样品分析要求。结论:该方法操作简便、快速、灵敏度高,适合瑞舒伐他汀钙的临床药代动力学研究。
AIM: To establish a rapid and sensitive LC-MS/MS method for the analysis of rosuvastatin calcium in plasma and study the pharmacokinetics of rosuvastatin on China healthy volunteers. METHODS: Plasma samples containing rosuvastatin calcium and fluvastatin sodium were extracted with liquid-liquid extraction, followed by liquid chromatographic separation and on-line MS/MS using turboionspray ionization as an interface detection by the muhi-reaction monitoring. RESULTS: The limit of quantitation of method for rosuvastatin calcium was 0.03 ng/mL, the calibration curves in plasma were linear over the range of 0.03 to 60 ng/mL and within-batch and between-batch variation over the range were less than 10%. CONCLUSION: The method is shown to be accurate and convenient, and suitable for pharmacokinetic studies of rosuvastatin calcium.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第7期819-823,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
瑞舒伐他汀钙
液相色谱-串联质谱法
药代动力学
血药浓度
rosuvastatin calcium
liquid chromatography tandem mass spectrometry
pharmacokinetics
plasma concentration