六种回收纯化差异显示PCR产物方法的比较

Comparison of Six Methods in Recovering Special Bangs in mRNA Differential Display

目的:建立mRNA差异显示PCR产物回收纯化方法。方法:采用了6种方法对干旱东亚砂藓mRNA差异显示技术的特异PCR产物进行分离回收纯化。结果:6种不同回收方法,显示不同的结果。结论:冻融法简单、方便、经济、有效,对东亚砂藓构建的mRNA差异显示技术中特异条带进行回收,具有有效性和可靠性。

Objective： To establish simplified methods to recover the special bands in the mRNA different display technique. Methods：There were six methods to recover the special bands in the technique of mRNA differem display of drought Racomitrium japonicum. Results： Six different recyclings methods, demonstrate the different result. Conclusion：The method of freeze - draw is simple, economy and effective for recovering the special bands in mRNA differential display.