甘薯(Ipomoea batatas L.)遗传转化几个因素的研究

Study on Factors Influencing Efficiency of Sweetpotato Transformation

目的：提高甘薯的遗传转化率,为建立快速高效的甘薯遗传转化体系奠定基础。方法：以甘薯多个优良栽培品种无菌苗茎切段为受体,利用农杆菌LBA4404/pSP10Z做介导,研究了影响甘薯转化的几个因素。结果：接种侵染时间对转化效率影响很大,以2-15min为宜,最高转化率可达32.14%;不同基因型的转化受体之间转化率差别较大;在培养过程中最后30min加入终浓度为50μmol/L AS的接种菌侵染的外植体,转化率较对照提高了2.02倍;共培养培养基中加入终浓度为50μmol/L的AS,转化率较对照组提高了6.11倍;AS终浓度为50μmol/L同时pH为4.8的共培养培养基有利于转化的发生,转化率较对照组提高了1.73倍;共培养培养基中脯氨酸的添加并不能提高转化率。结论：该研究为快速高效甘薯遗传转化体系的建立提供了依据。

Objective： Sweetpotato transformation efficiency would been raised. Methods： In this paper, the stem explants of many superior cultivats of sweetpotato and LBA4404/pSP10Z strain of Agrobacterium tumefaciens were used as test material. The effect of many factors on sweetpotato genetic transformation had been studied. Results： Inoculation duration had effect on efficiency of transformation. The suitable time for inoculation duration was 2- 15min. The highest fiequency of transformation was 32.14%. The transformed receptors of different genotypes had different sensitivity to the same Agrobacterium strain. Transformation efficiency had been raised 2.02 times when 50μmol/L acetosyringone was presence in culture of bacterium at the last 30min. Transformation efficiency had been raised 6.11 times when 50μmol/L acetosyringone was presence in co - culture medium. The pH 4.8 of co- culture medium could raise transformation efficiency too. The transformation efficiency had been raised 1.73 times. Conclusion： This paper supplied some gist for the establishinent of fast and efficient genetic transformation system of sweetpotato mediated by A. tumefacienens.