摘要
采用组织培养方法,设置不同激素配比的培养基对水蔓菁(Veronica linariifoliasubsp.dilatata)不同外植体叶片、茎尖及茎段进了行研究。结果表明MS+6-BA 2.0 mg/L+NAA 0.5 mg/L诱导愈伤组织效果最佳,其中以茎尖的诱导效果优于其他外植体;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L诱导愈伤组织丛生芽的分化效果最佳;1/2 MS+IBA 0.5 mg/L诱导丛生芽的生根效果最佳。
To establish a high efficiency plant regeneration system of Veronica linariifolia., callus, rosette buds, and roots were induced by different combinations of hormone from leaves, stem-tips and stems of Veronica linariifolia. The results showed that the medium of MS＋ 6 - BA 2.0 mg/L＋ NAA 0.5 mg/L was the best for callus induction, the medium of MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.05 mg/L was the best for bud differentiation, and the medium of 1/2 MS＋ IBA 0.5 mg/L was the best e for rooting.
出处
《四川农业大学学报》
CSCD
2007年第2期117-120,共4页
Journal of Sichuan Agricultural University
基金
四川农业大学青年科技创新基金。
关键词
水蔓菁
组织培养
植株再生
Veronica linariifolia
tissue culture
plant regeneration
