草鱼幼鱼肌醇营养需要量的研究

Dietary myo-inositol requirement for grass carp,Ctenopharyngodon idella fingerling

以酪蛋白、明胶和鱼粉为蛋白源,配制含肌醇水平分别为0、50 mg/kg、100 mg/kg、200 mg/kg、400 mg/kg8、00 mg/kg、1 600 mg/kg的7组实验饲料。每组设3个重复,连续投喂体质量(4.78±0.18)g的草鱼(Ctenopharyngodon idella)幼鱼9周,通过测定生长指标、部分血清生化指标和全鱼营养成分来评价饲料肌醇添加水平对草鱼幼鱼的影响。结果表明,饲料中肌醇添加水平≥200 mg/kg使草鱼幼鱼增重率(WGR)、特定生长率、血清中总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量与对照组相比有显著提高(P<0.05),而血清甘油三酯(TG)含量比对照组有显著降低(P<0.05);200 mg/kg和400 mg/kg肌醇添加组草鱼幼鱼饲料系数(FCR)比对照组有显著降低(P<0.05);饲料中添加肌醇对草鱼幼鱼存活率、血清中高密度脂蛋白胆固醇和全鱼营养成分无显著影响(P>0.05)。对WGR、FCR、TC、TG和LDL-C进行折线回归分析得出饲料中肌醇添加水平为166～214 mg/kg对草鱼幼鱼的生长比较适宜。

An 9-week growth experiment was conducted to estimate the requirement of myo-inositol （MI） for grass carp, Ctenopharyngodon idella fingerling. Half-purified basal diets were formulated using the fish meal, glutin and casein as the protein source. MI was supplemented at 0, 50,100,200,400,800, and 1 600 mg/kg diet in the basal diet. Each diet was fed to triplicate groups of grass carp with initial average body weight （4.78 ± 0.18）g. Grass carp fingerling fed diets supplemented with ≥200 mg MI/kg had significantly higher weight gain, specific growth rate, total cholesterol and low density lipoprotein cholesterol in serum than the control group fed diet without M I supplement（P 〈 0.05）. In contrast, the test groups had significantly lower triacylglycerol in serum than the control group（ P （0.05）. Also grass carp fingerling fed diets with 200 mg （MI）/kg and 400 mg （MI）/kg had significantly lower feed conversion rate than the control group（P 〈0.05）. Supplementation of dietary MI had no effect on survival, high density lipoprotein cholesterol in serum and the whole fish biochemical composition of grass carp fingerling （P 〉0.05）. Weight gain rate, feed conversion rate, total cholesterol, triacylglycerol and low density lipoprotein cholesterol of grass carp analyzed by broken-line regression indicated that the suitable requirement for dietary MI of growing grass carp was approximately 166- 214 mg/kg diet. [Journal of Fishery Sciences of China,2007,14 （5） ：794 - 800]