施万细胞对共培养的神经干细胞增殖与分化的影响

Effects of Schwann Cells on the generation and differentiation of neural stem cells in vitro

目的:探讨施万细胞诱导神经干细胞增殖与分化的影响因素。方法:大鼠胚胎神经干细胞单独培养及与新生SD大鼠施万细胞共培养,观察神经干细胞的形态变化,并分别检测特异性标志蛋白S-100、NF和GFAP的表达。结果:相差显微镜下可见共培养组大部分神经干细胞伸出多个细长突起;与单独培养组相比,免疫荧光染色显示共培养组NF表达阳性细胞数多;GFAP表达阳性细胞数少。结论:施万细胞可促进共培养的神经干细胞增殖,并可诱导神经干细胞向神经元方向分化。

Objective：To explore the influencing factors of Schwann Cells in promoting neural stem cells to generate and differentiate in vitro.Methods：Neural stem cells were dissociated and cloned from the cerebral subventricular zone of embryo rats,Schwann Cells were also dissociated and purified simultaneously from the sciatic nerves of newborn rats.Then neural stem cells were cultured separately and Co-cultured with Schwann Cells.The expression of protein S-100, neurofilament protein （NF） and glial fibrillary acidic protein （GFAP） of the neural stem cells were detected by immunofluorescence. The morphological changes of neural stem cells were examined with contrast phase microscope.Results：The co-cultured groups had more positive Cells of protein S-100 and NF,and less positive cells of GFAP than the group cultured separately. The nervous process of neuron-like cells in Co-cultured groups were obviously longer and more than that in separately cultured group.Conclusion：Schwann Cells can promote the generation and differentiation of neural stem cells in vitro.