摘要
采用流变学的方法研究胶原在受到一定剪切作用时的热稳定性能。试验结果表明:受剪切速率为1s-1的剪切作用后,胶原的热变性温度为31.1℃,比用乌氏黏度法测得的热变性温度低约4℃。从聚丙烯酰胺凝胶电泳、圆二色谱和原子力显微镜的试验结果可以看出:胶原加热到33℃恒温30min后,仍然能够保持其天然的结构,但是在33℃剪切处理30min后,胶原的相对分子质量变小而且分子质量分布变宽。圆二色谱显示在221nm的正吸收强度减弱,所成纤维没有周期性明暗相间的条纹,表明33℃时受剪切作用后的胶原的天然三股螺旋结构已经被部分破坏。由此说明剪切作用可在一定程度上使胶原的热稳定性能降低。
The denaturation temperature of collagen sheared at certain shear rate was studied. It shows that when the shear rate is 1s^-1 it , the denaturation temperature is 31.1℃ and is 4℃ lower than that determined by Ubbelohde viscometric measure- ment. The results of sodium dodecyl sulphate - polyacrylamide gel electrophoresis （ SDS - PAGE）, circular dichroism （CD） and atomic force microscopy （AFM） show that when collagen is heated at 33℃, it could keep its natural structure. However, the mo-lecular weight was smaller and the distribution is wider after the collagen is sheared at 33℃ for 30 min. The positive maximum value of molar ellipticity at 220 nm decreases in CD spectrum, and the AFM image presents the fibrils are irregular without a hint of periodicity, indicating the collapse of the triple helical configuration of collagen.
出处
《中国皮革》
CAS
北大核心
2007年第17期12-15,共4页
China Leather
基金
国家自然科学基金资助项目(20576083)
关键词
胶原
剪切
热变性温度
collagen
shearing
denaturation temperature
