低温弱光胁迫对野生大豆和大豆栽培种光系统功能的影响

Effects of Chilling Stress under Weak Light on Functions of Photosystems in Leaves of Wild Soybean and Cultivatar Soybean

以野生大豆和栽培大豆为材料,通过同时测定大豆叶片的叶绿素荧光快速诱导动力学曲线和对820nm光的吸收曲线,以及测定超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)的活性,分析了低温弱光胁迫及常温弱光恢复下这2种大豆光系统II(PSII)和光系统I(PSI)功能的变化。结果表明,低温弱光胁迫对这2种大豆的PSI和PSII的功能都造成伤害;在低温弱光胁迫下,维持较高的SOD和APX活性和维持PSI和PSII的协调性是野生大豆比栽培大豆耐低温的一个重要原因。

By simultaneously analyzing chlorophyll a fluorescence transient （Fig.1） and light absorbance at 820 nm （Fig.4）, and detecting activities of superoxide dismutase （SOD） and ascorbate peroxidase （APX）, the effects of chilling stress under weak light on functions of photosystem I （PSI） and photosystem II （PSII） in leaves of a wild type soybean variety ＇ZYD00019＇ and a cultivar soybean variety ＇Zaoshu No. 1＇ were investigated. The results showed that the functions of both PSI and PSII were damaged by the chilling stress under weak light （Figs.2-4）. The higher tolerance tochilling stress under weak light in the wild soybean might be due to its higher activities of SOD and APX （Fig.6） and its stability and coordination of its PSI and PSII （Figs.4,5）.