摘要
目的:将TFPI-2基因转染胰腺癌细胞系Panc-1细胞,研究其对胰腺癌细胞凋亡的影响.方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况.结果:同转染空载体及未转染细胞相比,转染成功的Panc-1细胞可以检测到TFPI-2 mRNA和相应蛋白的表达,细胞生长受到抑制(n=9,P=0.02),DNA琼脂糖凝胶电泳显示凋亡细胞特有的"梯状"条带.结论:外源性TFPI-2基因能够抑制胰腺癌细胞生长增殖、诱导胰腺癌细胞凋亡.
AIM: To study the effect of the TFPI-2 gene on apoptosis of human pancreatic cancer cells.
METHODS: Recombinant pEGFP-C1-TFPI-2 was transfected into Panc-1 cells using liposomes. Positive cells were selected by G418. Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT- PCR) and Western blotting. Cell growth and apoptosis were also studied.
RESULTS: Expression of TFPI-2 mRNA and protein was detected in Panc-1 cells transfected with pEGFP-C1-TFPI-2. Comparing Panc-1 cells transfected with vector and untransfected cells, the growth rate was lower and the apoptotic index of G418-resistant Panc-1 cells with the expression of TFPI-2 protein was faster(n = 9, P= 0.02).
CONCLUSION: The TFPI-2 gene can inhibit the growth of Panc-1 cells and cause cells to enter apoptosis.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第19期2093-2096,共4页
World Chinese Journal of Digestology
基金
安徽省自然科学基金资助项目
No.050430702