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TFPI-2基因体外诱导胰腺癌Panc-1细胞的凋亡 被引量：1

Apoptosis of Panc-1 pancreaticcancer cells induced by the TFPI-2 gene in vitro
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摘要 目的:将TFPI-2基因转染胰腺癌细胞系Panc-1细胞,研究其对胰腺癌细胞凋亡的影响.方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况.结果:同转染空载体及未转染细胞相比,转染成功的Panc-1细胞可以检测到TFPI-2 mRNA和相应蛋白的表达,细胞生长受到抑制(n=9,P=0.02),DNA琼脂糖凝胶电泳显示凋亡细胞特有的"梯状"条带.结论:外源性TFPI-2基因能够抑制胰腺癌细胞生长增殖、诱导胰腺癌细胞凋亡. AIM： To study the effect of the TFPI-2 gene on apoptosis of human pancreatic cancer cells. METHODS： Recombinant pEGFP-C1-TFPI-2 was transfected into Panc-1 cells using liposomes. Positive cells were selected by G418. Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction（RT- PCR） and Western blotting. Cell growth and apoptosis were also studied. RESULTS： Expression of TFPI-2 mRNA and protein was detected in Panc-1 cells transfected with pEGFP-C1-TFPI-2. Comparing Panc-1 cells transfected with vector and untransfected cells, the growth rate was lower and the apoptotic index of G418-resistant Panc-1 cells with the expression of TFPI-2 protein was faster（n = 9, P= 0.02）. CONCLUSION： The TFPI-2 gene can inhibit the growth of Panc-1 cells and cause cells to enter apoptosis.
作者 汤志刚 孙振阳 胡何节 许戈良 陈炯 李建生
机构地区 安徽医科大学附属安徽省立医院普通外科
出处 《世界华人消化杂志》 CAS 北大核心 2007年第19期2093-2096,共4页 World Chinese Journal of Digestology
基金 安徽省自然科学基金资助项目 No.050430702
关键词 胰腺肿瘤 细胞凋亡 组织因子途径抑制物2 免疫印迹 逆转录-聚合酶链反应 Pancreatic neoplasms Apoptosis TFPI-2 Western blotting Reverse transcriptionpolymerase chain reaction
分类号 R735.9 [医药卫生—肿瘤]
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参考文献30

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同被引文献30

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世界华人消化杂志
2007年 第19期

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