pp32表达引起人肝癌细胞HepG2形态改变

Expression of pp32 in human hepatoma cell line HepG2 leads to morphological changes

目的:研究pp32在肝癌细胞中的作用。方法:采用RT-PCR的方法钓取pp32全长基因。脂质体介导的方法将pp32基因导入HepG2细胞。观察细胞形态,血清依赖性和接触抑制等细胞表型,测量细胞生长曲线,检测ALP、LDH、γ-GT、ALB和AFP等生化指标。结果:成功钓取了pp32全长基因,筛选获得pp32稳定表达的HepG2细胞株。pp32表达使HepG2细胞伸出细长突起,但不影响HepG2细胞增殖。ALP和LDH酶活力升高,其他指标没有变化。结论:pp32表达引起人肝癌细胞HepG2形态改变,但不改变其恶性表型。

Objective： To study the function of pp32 in hepatoma cells. Methods ：The pp32 gene was amplified by RT- PCR and transfected into HepG2 cells using liposome. The morphology, serum dependence, contact inhibition and growth curve of pp32-stably expressed HepG2 cells, and other biochemical parameters such as ALP, LDH,γ- GT, ALB and AFP were observed. Results：Full length gene of pp32 was obtained and pp32-expressing HepG2 cell line was established. Con- stitutive expression of pp32 induced morphological changes and increase in ALP and LDH activity. But no proliferation or other biochemical parameters were changed. Conclusion： Expression of pp32 in human hepatoma cell line HepG2 leads to morphological changes, but not malignant transformation.