小鼠脾脏树突状细胞的分布与形态学观察

Murine spleen dendritic cells:an observation of their morphology, content and distribution

目的:认识脾脏树突状细胞的含量、分布及形态特征。方法:运用光镜、电镜观察,免疫组化标记(CD11c、CD205、CD80与CD86、I-Ab)与免疫荧光标记方法,观察小鼠脾脏树突状细胞的含量、分布与形态结构。结果:小鼠脾脏白髓与红髓中散在分布着体积较大的不规则形树突状细胞,含量以白髓边缘区最多,依次为白髓和红髓脾索。CD11c+DC约占脾脏细胞总数的3%,而CD205+DC约占脾脏细胞总数的2‰。脾脏树突状细胞表面形成数个胞浆突起。胞核略长多有凹陷。胞浆低电子密度,细胞器不发达,无溶酶体和吞噬体。结论:多种树突状细胞标志物联合标记,结合光、电镜形态学观察,能够较全面反映小鼠脾脏内树突状细胞的形态分布特征,为树突状细胞研究提供了形态学依据。

Objective：To observe the morphology, content and distribution of murine spleen dendritic cells （DCs）. Methods：Murine spleen dendritic cells were observed and analyzed by light microscopy, electron microscopy, immunofluorescence and the immunohistochemistry technique with DC markers including CD11 c, CD205, CD80, CD86 and I-A^b. Resuits： There were some big cells with irregular shapes scattered in the white pulps and red pulps and the DCs were mainly distributed in the marginal zone of the spleen cortex and in the white pulps and spleen cords of red pulps. The content of CD11c^＋ DCs in the spleen was about 3% of all spleen cells, whereas the content of CD205^＋ DCs was about 2‰ . On the surfaces of DCs there were several protrusions. The nuclei were somewhat long and there were indentations on the surface of them. The cytoplasm was electron-lucent with some organelles, but the lysosome and the phagosome, were not seen. Conclusion： Several DC markers combined with light microscopy and electron microscopy observation could well reflect the characteristics of the distribution and morphology of the DC, and could provide a morphology basis for the DC study.