莱茵衣藻-se两步法产氢用培养基的研究

Studies on the formulation of culture medium for production of hydrogen with Chlamydomonas reinhardtii-se

分析了TAP(Tris-Acet-Phosphate)、seTA(se-Tris-Acet)、seP(se-Phosphate)、seTH(se-Tris-HCl)和se这5种培养基对莱茵衣藻-se生长产氢的影响,并进行了培养基的含量分析.结果发现,培养基的pH和氮素的含量是影响莱茵衣藻生长的重要因素;而培养基中醋酸根的含量则有助于提高厌氧产氢过程中衣藻的氢酶活性,从而提高其产氢量.综合考虑两步法产氢对细胞生长周期和细胞密度的要求以及最终厌氧诱导获得的氢酶活性,最终选择seTA培养基作为莱茵衣藻-se产氢用培养基.

Chlamydomonas reinhardtii-se with longer growth cycle different from other wild strains used broadly before has been chosen in the two-stage photosynthesis and H2 production progress. Using the length of cells＇ growth cycle, the density of cells and the activity of induced hydrogenase as criteria, the optimal formulation of culture medium was obtained as seTA（se-Tris-Acet） which contains acetic acid as C source, higher concentration of N and stronger pH buffer system based on the se formulation. The results also indicate that the pH stability and the concentration of N exert a great influence on the growth of C reinhardtii-se and the acetic acid is the key substance for hydrogenase induction.