Dot-ELISA联检牛羊捻转血矛线虫和肝片吸虫病的研究

Studies on the simultaneous diagnosis of haemonchsis and fascioliasis in cattle and sheep by Dot-ELISA

应用Ｄｏｔ－ＥＬＩＳＡ联检１４２００头牛羊捻转血矛线虫和肝片吸虫病抗体，其中５５头羊与剖检捻转血矛线虫和肝片吸虫虫体的比较，其阳性符合率均达１００％，并可测出自然感染１～２条以上的捻转血矛线虫和肝片吸虫病抗体；３７３头（牛３１０头，羊６３头）血检阳性的牛羊与粪检的比较，其捻转血矛线虫和肝片吸虫病的阳性符合率分别达９２７９％和９５１５％。

The antibodies of Haemonchus contortue and Fasciola hepatica from a group of 14,200 cattle and sheep was simultaneously examined by Dot-ELISA The Dot-ELISA data were compared with the results of the autopsy in 55 sheep tested The positive specificities of the Dot-ELISA for both H contortue and F hepatica were 100% The Dot-ELISA was able to detect the antibodies from the animals naturely infected with 1～2 H contortus or F heptica 373 cattle and sheep with postive results by the Dot-ELISA were examined for faecal eggs The positive specificities were 9279% and 9515% for H contortus and F hepatica, respectively The results indicated that the Dot-ELISA will be a useful tool for diagnosis of Haemonchosis and Fascioliasis in cattle and sheep