摘要
对杨树无性系ＮＬ－８０１０５，ＮＬ－８０１０６，ＮＬ－８０１１７和湿地松组织培养愈伤组织的耐盐性进行了研究。结果表明：以ＭＳ为基本培养基，添加２，４－Ｄ２．０ｍｇ·ｋｇ－１和ＫＴ０．５ｍｇ·ｋｇ－１，诱导这些树种愈伤组织效果好；在含氯化钠的培养基上，杨树不同器官外植体的愈伤组织诱导能力和耐盐程度有差异，外植体茎、叶比根强；杨树继代愈伤组织的耐盐能力显著高于湿地松，这与植株水平的耐盐性相一致。
The tolerance to NaCl of tissues culture calli of Populus clones(NL 80105,NL 80106,and NL 80117) and Pinus elliottii was studied The results were as follows:(1) MS medium with 2 0 mg·kg -1 of 2,4 D and 0 5 mg·kg -1 KT was suitable for tree calli in tissue cultures;(2) the salt tolerances of calli from roots,stems and petioles were different on MS containing NaCl The calli with the highest salt tolerance was from stems;(3)the salt tolerance of subculture calli of Populus clones was higher than that of Pinus elliottii,which was in keeping with the salt tolerance of Populus and Pinus elliottii trees
出处
《浙江林学院学报》
CSCD
1997年第1期16-21,共6页
Journal of Zhejiang Forestry College
基金
浙江省自然科学基金
关键词
杨属
湿地松
组织培养
愈伤组织
耐盐性
Populus
clones
Pinus elliottii
tissue cultures
callus
salt tolerance
