RP-HPLC同时测定苯巴比妥、苯妥英和卡马西平的血药浓度(英文)

Simultaneous determination of phenobarbitone,phenytoin and carbamazepine in human plasma by RP-HPLC

目的采用反相高效液相色谱法同时测定苯巴比妥(PB)、苯妥英(PHT)和卡马西平(CBZ)血药浓度。方法色谱柱为Agilent Zorbax 8 nm Extend-C_(18)(5μm,4.6mm×150mm),流动相为V(甲醇):V[V(0.1mol/L乙酸钠):V(0.05%β-环糊精)=32:18]=68:32,柱温为室温,流速为0.8 mL/min,检测波长为230nm,进样量为20μL。结果PB、DPH和CBZ分离良好,分离度皆大于1.5;最低检测浓度分别为0.3802、0.1823和0.1444μg/mL;线性范围分别为0.4～60、0.2～30和0.2～30μg/mL;回收率分别为97.04%、96.52%和97.09%;日内和日间相对标准偏差皆小于5.80%。结论本方法简便、快速、重现性好,结果可靠,适用于临床常规检查。

Objective To establish a method for the determination of phenobarbitone （PB）, phenytoin （PHT） and carbamazepine （CBZ） in human plasma by reversed-phase HPLC at the same condition. Methods Agilent Zorbax 8 nm Extend-Cls （5 #m, 4.6 mmx150 mm） was used as analytical column and a mixture of V（methanol） ： V[V（0.1 mol/L sodium acetate） ： V（0.05% β-cyclodextrin）=32 ： 18]=68 ： 32 as mobile phase. The UV wavelength was 230 nm and flow rate was 0.8 mL/min. Results PB, PHT and CBZ were separated well. Resolving powers were all more than 1.5. The lowest detective concentrations of PB, PHT and CBZ were as following： 0.3802, 0.1823 and 0.1444 μg/mL. The linear ranges were in the range of 0.4-60, 0.2-30, 0.2-30 μg/mL, and relative recovery rates were 97.04%, 96.52% and 97.09% respectively. The relative standard deviations of within-day and between-day were all less than 5.8%. Conclusion The method is simple, sensitive, convenient and reliable. It is suitable for the determination of phenobarbitone, phenytoin and carbamazepine in human plasma at clinic as normal test.