大孔树脂柱层析法纯化金樱子总黄酮的初步研究

Study on Purification of Total Flavonoids from Rosa Laevigata with Macroporous Resin Column Chromatography

目的:研究AB-8大孔树脂纯化金樱子总黄酮的工艺条件。方法:用70%乙醇提取金樱子中黄酮类化合物,得到粗黄酮粉,利用分光光度法测定样品中的黄酮含量。以AB-8型大孔吸附树脂对金樱子黄酮提取液的吸附为研究对象,对影响AB-8型大孔树脂动态吸附与解吸金樱子黄酮的各种因素进行了初步研究。结果:其最佳工艺为样液浓度0.3 mg/ml,上样量为120 mg、以1 ml/min吸附速率进行吸附,5BV的70%乙醇、1 ml/min的流速进行洗脱效果最佳。粗黄酮粉的黄酮含量为25.2%,经AB-8型大孔吸附树脂纯化后的金樱子精黄酮粉中黄酮的含量达42.2%。结论:AB-8大孔吸附树脂对金樱子黄酮有一定的纯化效果。

Objective：To Study the technologic conditions of purifying total Rosa laevigata by AB-8 pocket. Methods：Extracting flavonoids from Rosa laevigata with 70% alcohol, the thick flavonoid powder were obtained and the content of pure flavonoids in it could he determinated using spectrophotometry. Taking the adsorption of the AB-8 pocket to Rosa flavonoids as the research object, the factors that affected the AB-8 resin static absorption and desorption were studied preliminarily. Results：The result showed that the effect was perfect when the flavononid concentration in the liquid was 0. 3 mg/ml, the loading amount was 120 mg with the adsorption-power 1 mE/ min;And the volume of 70% alcohol was eluant 5 BV with desorption-power 1 mE/min. The pure flavonoid content in thick flavonoid powder was 25.2% and it reached 42. 2% after being purified by the AB-8 pocket adsorption resin. Conclusion：h proved AB-8 pocket adsorption resin have a better effect on purification of the flavononids of Rosa laevigata.