AgNO_3对苹果叶片培养的影响及其与乙烯产生的关系

EFFECT OF AgNO 3 ON APPLE LEAF CULTURE AND ITS RELATION TO ETHYLENE PRODUCTION

在附加６ＢＡ３０ｍｇ／Ｌ、ＩＢＡ００５ｍｇ／Ｌ的ＭＳ再生培养基中加入１～１００μｍｏｌ／ＬＡｇＮＯ３，均显著促进苹果叶片再生不定芽，但以１μｍｏｌ／Ｌ浓度效果最好。与对照相比，在培养早期（２～５ｄ），１μｍｏｌ／ＬＡｇＮＯ３对乙烯的抑制率为１６％～２０％，在培养中后期则为２５％～４０％，因而显著降低了各培养时期，特别是中后期（芽分化期）培养容器中乙烯的浓度，降低了乙烯对芽发生的抑制。

When AgNO 3(1～100μmol/L) was added to MS regeneration medium containing 6 BA 3 0ml/L,IBA 0 05mg/L,the shoot regeneration from apple leaf significantly enhanced.And the optimum concentration of AgNO 3 tested was 1μmol/L.In contrast to the control,the suppression rate to ethylene with 1μmol/L of AgNO 3 was 16%～20% in early culture stage(2～5days) while the rate was 25%～40% in middle and late stages.Therefore,the ethylene concentration in the container throughout the culture period especially at the middle and late stages(known as differentiation stage)decreased,thus lessening the ethylenes inhibition on shoot regeneration and promoting shoot formation.