大鼠骨髓间充质干细胞条件培养液促进心肌细胞肥大

Cardiomyocyte Hypertrophy Induced by Cultured Rat Mesenchymal Stem Cells Conditioned Medium

体外模拟心肌缺血微环境,研究骨髓间充质干细胞(MSCs)的旁分泌作用对心肌细胞的影响。以大鼠MSCs各时间点的条件培养液刺激心肌细胞,观察心肌细胞蛋白含量、[3H]-Leu掺入、ANF-荧光素酶(luciferase)表达和心肌细胞面积的变化。MSCs条件培养液处理心肌细胞后,与对照组相比较6h及9h时间点的条件培养液可明显增加心肌细胞蛋白含量、[3H]-Leu掺入、ANF-荧光素酶表达以及心肌细胞面积,其中以6h时间点条件培养液的作用最为显著(P<0.01)。MSCs条件培养液能够通过旁分泌作用刺激心肌细胞肥大,此现象提示移植入心肌缺血区MSCs可能通过旁分泌作用影响心肌细胞,从而参与细胞移植后心功能的改善。

This study examined the hypertrophic effect of mesenchymal stem cells-conditioned medium （MSCs-CM） on neonatal rat cardiomyocytes. Rat MSCs were cultured with serum-free medium under hypoxic condition for 3 h, 6 h, 9 h and 24 h, and then the conditioned medium were collected separately. Cultured neonate rat cardiomyocytes were examined by ^3[H]-leucine incorporation, ANF-luciferase expression, F-actin staining and cell area measurement after stimulated with the above MSCs-CM for 48 h. Compared with control group, the total protein content, ^3[H]-leucine incorporation, ANF-luciferase expression and cell area were obviously increased in MSCs-CM stimulated groups. These results demonstrated that MSCs-CM induced cardiomyocyte hypertrophy in a paracrine manner and implied that this effect likely involved in the improvement of heart function after MSCs transplantation.