痰标本中肺炎克雷伯菌超广谱β-内酰胺酶基因的检测及耐药分析

Study of Klebsiella pneumonia isolated from the sputum on the gene typing of extended-spectrum β-lactamases and antibiotic resistance

目的分析痰标本中肺炎克雷伯菌的耐药特点及超广谱β-内酰胺酶(ESBLs)的发生率、基因分型。方法收集安徽省内34家医院痰标本中分离到的114株肺炎克雷伯菌,用琼脂稀释法检测其耐药性,标准纸片扩散法及PCR法检测ESBLs基因并初步分型。结果114株肺炎克雷菌中产ESBLs58株(50.9%),其中CTX-M-1型12株,CTX-M-9型15株,TEM型42株,SHV型49株,OXA-1型2株;产ES-BLs肺炎克雷伯菌的耐药率明显高于非产ESBLs菌株。结论痰标本中产ESBLs肺炎克雷伯菌检出率高,且呈多重耐药。

Objective To investigate the antibiotic resistance and genotype distribution of extended-spectrum β- lactamases（ESBLs） producing Klebsiella pneumonia isolated from the sputum. Methods A total of 114 strains of Klebsiella pneumonia were chosed from 34 different hospitals in Anhui province. Agar dilution test was used in susceptibility testing. The ESBLs producers were detected by standard disk diffusion test. In the further test, initial typing of ESBLs was conducted by PCR. Results 58 strains of Klebsiella pneumonia producing ESBLs were deteced ,including CTX-M-1 （ 12 strains ）, CTX-M-9 （ 15 strains ）, TEM （42 strains ）, SHV （49 strains ）, OXA-1 （2 strains）. The resistant rates of strains producing ESBLs were much higher than that of non-ESBLs-producing strains. Conclusion The Klebsiella pneumonia producing ESBLs isolated from the sputum are common, and most of them are mutiple drug resistant.