野菊花总黄酮对四氯化碳致急性肝损伤小鼠的保护作用

The protective effects of total flavonoids Chrysanthemum indicum on CCl_4-induced acute liver injury in mice

目的观察野菊花总黄酮(TFC)对CCl4致小鼠急性肝损伤的保护作用及部分机制。方法用TFC(125、250、500 mg/kg)连续给小鼠灌胃4后,腹腔注射0.1%CCl4(10ml/kg)致小鼠急性肝损伤模型,测定不同剂量的TFC对肝损伤血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)活性、肝匀浆中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的影响;并通过放射免疫分析法测定肝匀浆中细胞因子肿瘤坏死因子-α(TNFα-)的表达量;同时对肝组织进行病理学检查。结果TFC能降低CCl4致小鼠急性肝损伤血清ALT、AST值的升高,降低肝匀浆中MDA含量,增强SOD的活性,TNFα-表达降低,减轻CCl4对肝组织的病理损伤。结论TFC对CCl4致小鼠急性肝损伤具有一定的保护作用,其机制可能主要与清除自由基、抑制脂质过氧化作用、抑制TNFα-表达有关。

Objective To study the protective effects and mechanism of total flavonoids Chrysanthemum indicum （TFC） on CCl4-induced acute liver injury in mice. Methods The animal model of acute liver injury was established by 0. 1% CCl4 （10 ml/kg, ip）. To evaluate the hepatic injury of these animals, we observed changes of hepatocytes through （pathological section ,tested the serum transaminases（ ALT, AST） activity, and detected hepatic tissue MDA contents and SOD activities. The mechanism of TFC on acute liver protection was analyzed the expression of TNF-α by the method of Radioimmunoassay. Results TFC could significantly decrease the activities of serum ALT and AST. MDA contents dropped markedly and SOD activities increased greatly. Pathohistological observation indicted the liver lesion was markedly alleviated. The liver protective mechanism of TFC might be related with decreased expression level of TNF-.α Conclusion TFC has protective effects on CCl4-induced acute chemical liver lesion, the mechanism might be related with scavenging free radical, resisting lipid peroxidation and decreasing expression level of TNF-α.