膀胱移行细胞癌中促红细胞生成素及其受体的表达

Expression of erythropoietin and its receptor in transitional cell carcinoma of bladder

目的探讨促红细胞生成素(EPO)及其受体(EPOR)在膀胱移行细胞癌(TCC)组织中的表达,并探讨其临床意义。方法应用免疫组织化学方法、RT-PCR方法检测70例膀胱TCC组织、15例癌旁组织、10例正常膀胱组织中EPO和EPOR的蛋白和 mRNA的表达。结果70例膀胱TCC组织有44例(62.9%)EPO蛋白阳性表达,有29例(41.4%)EPOR蛋白阳性表达;15例膀胱TCC癌旁组织及10例正常膀胱组织均未见EPO、EPOR蛋白的表达。EPO蛋白表达强度与膀胱TCC的组织学分级相关(rs=0.329,P<0.05),而与临床分期则无明显的相关性。膀胱癌组织中EPO与EPOR表达具有相关性(rs=0.346,P<0.01)。RT-PCR检测发现膀胱移行细胞癌组织中EPO及其受体 mRNA的表达明显高于正常膀胱组织、癌旁组织中EPO及其受体 mRNA的表达(P<0.01)。结论EPO、EPOR在膀胱TCC组织中过表达,提示EPO、EPOR在膀胱TCC的发生、发展过程中可能起重要作用;EPO蛋白表达强度与膀胱TCC的组织学分级有明显相关性,可能成为膀胱TCC不良预后的指标之一。

Purpose To detect the expression of erythropoitin （EPO） and its receptor （EPOR） in the bladder transitional cell carcinoma （TCC） and normal bladder tissues, and its clinical significance. Methods Immunohistochemistry and RT-PCR were used to detect the expression of EPO and EPOR in 70 cases of bladder TCC tissue, 15 cases of adjacent benign tissue and 10 cases normal bladder tissue. Results 44 of 70 （62. 9% ） cases of bladder TCC were positive for EPO protein and 29 of 70 （41.4%） positive in EPOR protein, but none of positive expression of EPO and EPOR proteins were found in 15 adjacent benign tissues and 10 normal bladder tissues. There were significant differences of these values between bladder TCC and adjacent benign and normal bladder tissues （P 〈 0. 01 ）. The intensity of immunostaining in Ⅱ and Ⅲ degree bladder TCC were higher than I degree bladder TCC （ P 〈 0. 05 ）. RTPCR analyses found that the expression of EPO and EPOR mRNAs in bladder TCC tumor tissues were higher than those in the adjacent benign tissues and normal bladder tissues （ P 〈 0. 01 ）. Conclusions The over expression of EPO and EPOR is observed in the bladder TCC, indicating that EPO and EPOR may play an important role in carcinogenesis of human urinary bladder. The expression of EPO - is significantly associated with tumor grade. These results suggest that the expression of EPO may be considered as a prognostic factor for bladder TCC.