胃癌中肿瘤相关纤维母细胞蛋白表达改变及意义

Changes in protein expression and significance of tumorassociated fibroblasts in gastric cancer

目的：探讨胃癌中肿瘤相关纤维母细胞（tumor-associated fibroblasts，TAFs）蛋白表达的改变及与胃癌侵袭转移的关系．方法：应用免疫组织化学方法检测75例胃癌组织及10例正常胃黏膜组织中纤维母细胞CD34，平滑肌肌动蛋白（SMA）和转化生长因β1（TGF-β1）的表达，分析蛋白表达改变的机制及与胃癌临床病理特征的关系．结果：胃癌组织CD34，SMA和TGF-β1中的阳性表达与正常胃黏膜组织纤维母细胞中的表达（8．0％vs80．0％， x^2 =28．220；90．7％VS30．0％， x^2 =19．401；89.3％VS30．0％， x^2 =17．486；均P〈0．01）；SMA和TGF-β1均与与肿瘤浸润深度、临床分期和淋巴结转移有关（均p〈0．05），且两者的表达密切相关（r=0．555，P〈0．05）．结论：胃癌细胞可能通过分泌TGF-β1作用于间质，促进CD34（＋）纤维母细胞转变为SMA（＋）的肌纤维母细胞，从而发挥其促进肿瘤浸润转移的作用．

AIM： To study changes in protein expression in tumor-associated fibroblasts in gastric cancer and their relationship with tumor invasion. METHODS： We used immunohistochemistry to detect expression of CD34, smooth muscle actin （SMA） and transforming growth factor （TGF）-β1 in stromal fibroblasts of 75 gastric cancer and 10 normal gastric tissues, and analyzed its mecha- nism and relationship with clinical characteristics. RESULTS： There were statistically significant differences in expression of CD34, SMA and TGF-β1 between gastric cancer and normal gas-tric tissues （8.0% vs 80.0%, x^2 =28.220; 90.7% vs 30.0%, x^2 =19.401; 89.3% vs 30.0%, x^2 =17.486; P 〈 0.01, respectively）. Expression of TGF-β1 and SMA was related to depth of tumor, clinical stage and lymph node metastasis （both P 〈 0.05）. SMA was positively associated with TGF-β1 （r = 0.555, P 〈 0.05）. CONCLUSION： The loss of CD34 and the acquisition of SMA in tumor-associated fibroblasts are associated with gastric cancer invasion, and TGF-β1 may promote this phenotypic alteration.