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亚洲Ⅰ型口蹄疫病毒全长可读框的表达及其产物免疫原性分析 被引量：4

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摘要 将亚洲Ⅰ型口蹄疫病毒(foot-and-mouth disease virus,FMDV)全长约为6.9kb的可读框(ORF)克隆到家蚕杆状病毒转移载体pVL1393中,构建重组家蚕杆状病毒转移质粒pVL-ORF.pVL-ORF与线性化的亲本病毒Bm-BacPAK6 DNA共转染家蚕细胞,经空斑筛选后成功获得携带有FMDV全长ORF的重组病毒Bm-ORF.免疫荧光技术证明,重组病毒能够正确表达插入的外源基因.将获得的重组病毒Bm-ORF感染家蚕5龄起幼虫,双抗体夹心ELISA法和电子显微镜观察证明,目的基因在蚕体中获得较高水平表达并组装成空衣壳.用蚕表达产物作抗原制备疫苗免疫牛,免疫动物均产生了特异性抗体.免疫后21d进行病毒攻击保护实验,获得了2/4的完全保护.
作者 李志勇 易咏竹 殷相平 张志芳 柳纪省
机构地区 中国农业科学院兰州兽医研究所 中国农业科学院生物技术研究所
出处 《科学通报》 EI CAS CSCD 北大核心 2007年第16期1903-1907,共5页 Chinese Science Bulletin
基金 国家高技术研究发展计划(批准号:2006AA02Z440) 科技部社会公益研究专项(批准号:2005DIB4J041)资助项目
关键词 FMDV ORF 家蚕杆状病毒载体 免疫原性 疫苗
分类号 S852.65 [农业科学—基础兽医学]
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参考文献21

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二级参考文献29

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科学通报
2007年 第16期

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