反式二羟环氧苯并(a)芘所致人支气管上皮细胞POLK基因高表达

High expression of POLK gene in human bronchial epithelial cell induced by anti-BPDE

目的观察不同剂量反式二羟环氧苯并(a)芘(anti-BPDE)处理对人支气管上皮细胞(16HBE)POLK基因表达的影响。方法分别以0.25、0.50、1.00、2.00μmol/L的反式-BPDE1次或3次处理16HBE细胞,采用TaqmanMGB探针实时定量聚合酶链反应方法相对定量POLK和POLZ基因表达,同时对反式-BPDE转化的16HBE及肺癌H1299细胞POLK基因表达进行了分析。结果反式-BPDE1次处理及3次处理可诱导正常16HBEPOLK基因高表达。反式-BPDE转化16HBE和肺癌H1299细胞POLK基因表达明显增高,分别是正常16HBE的2.25和5.49倍。结论反式-BPDE处理可致16HBEPOLK基因高表达。

Objective To explore the effects of anti-BPDE on the expression of POLK gene in human bronchial epithelial cells. Methods 16HBE cells were treated by anti-BPDE at doses of 0. 25, 0. 50, 1.00, 2.00 μmol/L for one or three times. The expressions of POLK and POLZ genes were detected simultaneously with real-time RT-PCR using Taqman MGB probes. The expressions of these two genes in human lung cancer cells （H1299） and malignant transformed 16HBE cells induced by anti-BP- DE were also detected. Results The expressions of POLK gene in 16HBE cells were up-regulated induced by anti-BPDE at the dose of 0. 25 μmol/L for one time and 0. 25 or 0. 50 μmol/L for three times. The expressions of POLK gene in malignant transformed 16HBE cells induced by anti-BPDE and H1299 cells increased by 1.25 and 4.49 folds respectively, compared with the normal 16HBE. Conclusion High expression of POLK gene in human bronchial epithelial cells could be induced by anti-BPDE.