宫颈癌hMSH2、突变型P53及PTEN蛋白的异常表达

Abnormal expressions of hMSH2,mutated P53 and PTEN proteins in cervical carcinoma

[目的]探讨宫颈癌hMSH2、突变型P53及PTEN蛋白的异常表达在宫颈癌发病中的作用及临床意义。[方法]免疫组织化学SP法定位检测61例宫颈癌和19例慢性宫颈炎组织hMSH2、突变型P53及PTEN蛋白的表达情况。[结果]宫颈癌与慢性宫颈炎组,hMSH2蛋白阳性表达率分别为57.38%(35/61)和21.05%(4/19)(P<0.05);突变型P53蛋白阳性表达率分别为70.49%(43/61)和15.79%(3/19)(P<0.05);PTEN蛋白阳性表达率分别为59.02%(36/61)和26.32%(5/19)(P<0.05);三种蛋白共同表达率分别为29.51%(18/61)和5.26%(1/19)(P<0.05)。宫颈癌hMSH2蛋白表达阳性组与阴性组,突变型P53蛋白的阳性表达率分别为77.14%(27/35)、61.54%(16/26),差异无显著意义(P>0.05);PTEN蛋白的阳性表达率分别为62.86%(22/35)、53.85%(14/26),差异无显著意义(P>0.05)。宫颈癌突变型P53蛋白表达阳性组与阴性组,PTEN蛋白的阳性表达率分别为62.79%(27/43)、50.00%(9/18),差异无显著意义(P>0.05)。[结论]宫颈癌的发病过程中伴有hMSH2、突变型P53及PTEN蛋白表达上调,但三者高表达机制各自独立。3种蛋白联合检测有助于宫颈癌的预警和诊断。

[ Objective] To study the significance of abnormal expressions of DNA mismatch repair （ hMSH2）, mutated P53 and PTEN proteins in cervical carcinogenesis. [ Method] Immunohistochemistry SP method was used to detect the expressions of hMSH2 , mutated P53 and PTEN proteins in 61 cases, cervical carcinomas and 19 cases chronic cervicitises. [ Results] In cervical carcinoma and chronic cervicitis groups, the protein expression rates of hMSH2 were 57.38% and 21.05% （P 〈 0.05）; the rates of mutated P53 were 70.49% and 15.79% （ P 〈 0.05 ） ; the rates of PTEN were 59.02% and 26.32% （ P 〈 0.05） ; the rates of three proteins at the same case were 29.51% and 5.26% （P 〈 0.05 ）. In cervical carcinoma with the hMSH2 expression and without, the protein expression rates of mutated P53 were 77.14% and 61.54% respectively （ P 〉 0.05 ） ; the rates of PTEN were 62.86% and 53.85% respectively （ P 〉 0.05 ）. In cervical carcinoma with the Mutated P53 expression and without, the protein expression rates of PTEN were 62. 79% and 50.00% respectively （ P 〉 0.05 ）. [ Conclusion ] Following the cervical carcinogenesis, the expressions of hMSH2, mutated P53 and PTEN proteins increase independently. It may be helpful for predicting and diagnosing cervical carcinoma to detecting expressions of three proteins at the same time